Measurement of cultured cell adhesion
Başlık çevirisi mevcut değil.
- Tez No: 400242
- Danışmanlar: DR. BİLL WHİSH
- Tez Türü: Doktora
- Konular: Biyokimya, Biochemistry
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 1994
- Dil: İngilizce
- Üniversite: Unıversıty Of Bath
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Kimya ve Biyoloji Mühendisliği Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: Belirtilmemiş.
Özet
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Özet (Çeviri)
The involvement of cell adhesion in a very wide area from biotechnology to clinical applications made this area very attractive subject of study. To be able to study the underlying mechanism of cell adhesion, it is necessary to measure it. Most studies have been previously carried out qualitatively which will provide information whether cell-substratum compatible. However, a few quantitaive methods have been developed for the measurement of cell adhesion. While these suffer from a limited abilty or need of complex equipment.In the present work a simple and reproducible cell adhesion measuring device“Microflow chamber”has been developed which produces a wide range of hydrodynamic forces for cell detachment due to existence of convergent channel(s) in it. The reproducibility of this device was shown by determination of adhesion strength of different cell lines. Every cell line has a specific and constant adhesion strength.The requirement of metabolic energy from initial cell attachment to gaining maximum adhesion strength has been shown. The minimum serum concentration at which CHL cells are able perform adhesion fully was found to be 1%.The specificity of cell-substrate adhesion has been shown by determining that pre-adsorption of fibronectin and collagen type IV on tissue culture plastic dish strengthened dramatically while modification of these dishes with collagen type I did not make any significant difference on the adhesion of CHL cells. However, HeLa B cells were able to enhance their adhesion strength on collagen type I coated surfaces.The involvement of endogenous proteins in cell adhesion has been determined by inhibiting protein synthesis or secretion.
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