Spectral fluorescence measurements on reflecting surfaces shedding light onto conformation and orientation of macromolecules
Başlık çevirisi mevcut değil.
- Tez No: 400754
- Danışmanlar: PROF. SELİM ÜNLÜ
- Tez Türü: Doktora
- Konular: Elektrik ve Elektronik Mühendisliği, Electrical and Electronics Engineering
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2009
- Dil: İngilizce
- Üniversite: Boston University
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 134
Özet
Özet yok.
Özet (Çeviri)
The functionality of macromolecules depends strongly on their structural arrangements that form the functional sites and on the accessibility of these sites. These molecules are often immobilized on surfaces to study their interactions with other molecules, in biological and medical sciences. When macromolecules are in contact with a surface, a new structural arrangement, or conformation, may be adopted due to free energy minimization mechanisms, and the functional sites may be rendered inactive. Additionally, the molecular orientation acquired for surface attachment may hinder the accessibility of the functional sites. Therefore, conformation and orientation of macromolecules are of utmost importance to sustain their functional behavior on a surface and require systematic studies. However, the length scale necessary to directly measure macromolecular conformation is far beyond the capability of conventional viii optical microscopy. We have developed a spectroscopy technique to study the surface conformation and orientation of fluorescently tagged macromolecules. The technique, spectral selfinterference fluorescence microscopy (SSFM), utilizes the spectral oscillations in the radiated intensity due to the interference of direct and reflected fluorescence from emitters near a dielectric surface. Using the sub-nanometer emitter axial localization capability of this technique, we have determined the conformational change of surface adsorbed polymer chains upon hydration. The results revealed the importance of this conformational change in the efficiency of the polymeric structure for surface binding of probes in microarray applications to study ligand-analyte interactions. SSFM in the traditional configuration lacked high lateral resolution due to the low numerical aperture (NA) requirement to achieve sub-nanometer axial localization. However, for applications that involve resolving finer structures such as topographical mapping of cell surfaces or study of sub-cellular components, high lateral resolution is required. Using high-NA objectives results in a diminished fringe contrast of spectral oscillations, and thus the localization capability is reduced. Through angular tailoring of the radiated intensity, we have achieved sub-micron lateral resolution while maintaining sub-nanometer axial localization capability. The elimination of the low-NA requirement with this angular tailoring approach also opened up the possibility of determining the dipole orientation information from the spectral signature in addition to the axial position information. Simulations demonstrate that the dipole orientation of the emitters can be detected with a high precision.
Benzer Tezler
- Akridin esaslı boyaların ortak duyarlaştırıldığı güneş hücrelerinin fotovoltaik özelliklerinin incelenmesi
Investigation of photovoltaic properties of solar cells sensitized by acridine-based dyes
MELİHA GİZEM BEKMEZ
- Görece kısa süreli WiFi ışıması biyolojik dokuları etkiliyor mu?
Does relatively short term exposure to WiFi affect bi̇ologi̇cal ti̇ssues ?
YASEMİN MARAŞLIGİLLER
Yüksek Lisans
Türkçe
2014
Biyomühendislikİstanbul Teknik ÜniversitesiElektronik ve Haberleşme Mühendisliği Ana Bilim Dalı
PROF. DR. FATMA İNCİ ÇİLESİZ
- 60 KeV'lik fotonlarla uyarılmış Z=24-92 aralığındaki elementlerin K ve LX ışını floresans tesir kesitleri ve floresans verimleri
Başlık çevirisi yok
YÜKSEL ÖZDEMİR
Yüksek Lisans
Türkçe
1998
Fizik ve Fizik MühendisliğiAtatürk ÜniversitesiFizik Ana Bilim Dalı
YRD. DOÇ. DR. RIDVAN DURAK
- Alpagut- Dodurga (Osmancık-Çorum) bölgesi çevresindeki kömürlerin oluşum ortamları ve özelliklerinin belirlenmesi
Başlık çevirisi yok
SELAMİ TOPRAK
Doktora
Türkçe
1996
Jeoloji MühendisliğiHacettepe ÜniversitesiJeoloji Ana Bilim Dalı
PROF.DR. ERKAN YAVUZ
- Katkısız ve ZnO nanoparçacık katkılı NIPAM nanojellerininsentezlenmesi ve karakterizasyonu
Synthesis and characterization of undoped and ZnO nanoparticles doped NIPAM nanogels
OĞUZ GÜRCÜOĞLU
Yüksek Lisans
Türkçe
2018
Fizik ve Fizik Mühendisliğiİstanbul Teknik ÜniversitesiFizik Mühendisliği Ana Bilim Dalı
DOÇ. DR. ESRA ALVEROĞLU DURUCU