Geri Dön

Synthesis and use of single-walled carbon nanotube transistors for single-molecule studies of DNA polymerase I

Başlık çevirisi mevcut değil.

  1. Tez No: 402668
  2. Yazar: OSMAN TOLGA GÜL
  3. Danışmanlar: PROF. PHILIP G. COLLINS
  4. Tez Türü: Doktora
  5. Konular: Fizik ve Fizik Mühendisliği, Physics and Physics Engineering
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2014
  8. Dil: İngilizce
  9. Üniversite: University of California Irvine
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 121

Özet

Özet yok.

Özet (Çeviri)

The first half of this dissertation is focused on the synthesis of high quality single-walled carbon nanotubes (SWCNTs). The project goal was to improve SWCNT yields by tuning different growth conditions. Improvements in catalyst activity were observed with an H2O2 surface treatment and by tuning the hydrogen reduction time of the catalysts. In addition, a waterassisted chemical vapor deposition (CVD) was developed that used dry feedstocks with a small, controlled quantity of molecular oxygen. In this process, reactions between oxygen and hydrogen in the reaction zone provided precise water concentrations at the point of synthesis and short time constants. This water vapor dramatically increased SWCNT yield, but the added oxygen also changed the resulting SWCNT diameter distribution. SWCNTs were synthesized with a mean diameter of 0.7 nm and a narrow diameter distribution from 0.5 to 1.0 nm. Improvement in yield reached the limit where each particle resulted in one SWCNT. This allowed the catalyst concentration to be reduced by 102 to 103, greatly reducing catalyst contamination. In the second half of this dissertation, SWCNT field effect transistors (FETs) were used as single-molecule biosensors to investigate DNA polymerase I. DNA polymerases are critical enzymes for DNA replication, and because of their complex catalytic cycle they are excellent targets for investigation by single-molecule experimental techniques. The Klenow fragment (KF) of DNA polymerase I was studied by attaching single KF molecules to sensitive SWCNT FETs. Using these devices, this work investigated two aspects of KF activity. First, the kinetics of native dNTPs were compared to analog dNTPs. Analogs like α-thio-dNTP, 2-thio-dTTP, 2-thiodCTP, and 6-chloro-dGTP each proved that analogs had almost no effect on the duration of KF's closed conformation, the portion of the catalytic cycle during which a dNTP is incorporated. Instead, the entire effect of these analogs was to change the duration of the open conformation. The thiolated and chlorinated analogs appeared to interfere with KF's recognition and binding, two key steps that determine ensemble turnover rates. Second, KF devices were probed with small concentrations (

Benzer Tezler

  1. Synthesis and use of single-walled carbon nanotube transistors for single-molecule studies of dna polymerase i

    Tek duvarlı karbon nanotüp transistörlerin sentezlenmesi ve dna polimeraz ı tek-molekül çalışmalarında kullanılması

    OSMAN TOLGA GÜL

    Doktora

    İngilizce

    İngilizce

    2014

    BiyoteknolojiUniversity of California Irvine

    Fizik Ana Bilim Dalı

    PROF. DR. PHILIP G. COLLINS

  2. Sorting single wall carbon nanotubes by electronic structure using gel chromatography

    Tek duvarlı karbon nanotüplerin elektronik yapılarına göre jel kromatografi yöntemi ile ayrılması

    FERESHTEH ORDOKHANI

    Yüksek Lisans

    İngilizce

    İngilizce

    2016

    Kimyaİstanbul Teknik Üniversitesi

    Enerji Bilim ve Teknoloji Ana Bilim Dalı

    PROF. DR. NİLGÜN KARATEPE YAVUZ

  3. Co-Mo/MgO katalizörü ile yüksek verimli karbon nanotüp sentezi

    High efficiency carbon nanotube synthesis with Co-Mo/MgO catalyst

    AYŞEGÜL ÇAĞLA GERÇEK

    Yüksek Lisans

    Türkçe

    Türkçe

    2018

    Enerjiİstanbul Teknik Üniversitesi

    Enerji Bilim ve Teknoloji Ana Bilim Dalı

    PROF. DR. NİLGÜN YAVUZ

  4. Farklı destek ve katalizör malzemeleri ile tek duvarlı karbon nanotüp sentezi

    Synthesis of single walled carbon nanotubes with different substrats and catalysts

    FATİH GÜMÜŞ

    Yüksek Lisans

    Türkçe

    Türkçe

    2013

    Enerjiİstanbul Teknik Üniversitesi

    Enerji Bilim ve Teknoloji Ana Bilim Dalı

    PROF. DR. NİLGÜN KARATEPE YAVUZ

  5. Tek duvarlı karbon nanotüplerin organik güneş pillerinde elektrot malzemesi olarak kullanılması

    Carbon nanotube array electrodes for organic thin film

    OSMAN ÜRPER

    Yüksek Lisans

    Türkçe

    Türkçe

    2015

    Enerjiİstanbul Teknik Üniversitesi

    Enerji Bilim ve Teknoloji Ana Bilim Dalı

    PROF. DR. NİLGÜN KARATEPE YAVUZ