Geri Dön

Investigation of eradication of salmonella enteritidis and salmonella typhimurium biofilms using disinfectants and nanoparticles on food contact surfaces

Gıda temas yüzeylerinde dezenfektanlar ve nanopartiküller kullanılarak salmonella enterıtıdıs ve salmonella typhımurıum biyofilmlerinin ortadan kaldırılmasının araştırılması

  1. Tez No: 1019645
  2. Yazar: MELEK MELİSA ÖZGÖK
  3. Danışmanlar: PROF. DR. SEZA ARSLAN
  4. Tez Türü: Yüksek Lisans
  5. Konular: Biyoloji, Biology
  6. Anahtar Kelimeler: Biyofilmler, Dezenfektanlar, Eradikasyon, Nanopartiküller, Salmonella, Salmonella, Biofilm, Disinfectants, Nanoparticles, Eradication, Biofilms
  7. Yıl: 2026
  8. Dil: İngilizce
  9. Üniversite: Bolu Abant İzzet Baysal Üniversitesi
  10. Enstitü: Lisansüstü Eğitim Enstitüsü
  11. Ana Bilim Dalı: Biyoloji Ana Bilim Dalı
  12. Bilim Dalı: Biyoloji Bilim Dalı
  13. Sayfa Sayısı: Belirtilmemiş.

Özet

Biofilms are a constant source of contamination on food contact surfaces in the food industry and play a role in transfer of pathogens, primarily Salmonella, posing serious health problems. In this study, biofilm formation of Salmonella serovars Enteritidis and Typhimurium on polystyrene microtiter plates, stainless steel (SS), and polypropylene (PP) coupons as well as eradication of preformed Salmonella biofilms using peracetic acid (PAA) and sodium hypochlorite (SH) disinfectants and silicon dioxide (SiO2) and titanium dioxide (TiO₂) nanoparticles was examined. All 21 Salmonella isolates produced biofilm on coupons. SH treatment with MIC and sub-MIC at 1 and 5 minutes on SS and PP coupons showed a ≥3 log reduction in biofilms of all isolates (3 to 6 log). The reduction in PAA on SS and PP coupons at MIC and ½ MIC for 1 and 5 minutes was between 0.3-5.2 log and 0.1-6.5 log, respectively, indicating statistical significance (P ≤ 0.001). SiO₂ (0.2-3.8 log) and TiO₂ (0.1-5.1 log) at tested concentrations and times on PP showed a decrease of 1 log or more (>90% reduction) in approximately half of the isolates. SiO₂ (0.1-2.1 log) and TiO₂ (0.1 3.6 log) with MIC and ½ MIC for 5 minutes on SS were more effective, showing a 1 log or more reduction (>90%) than for 1 minute. There was no statistically significant difference between results of SiO₂ and TiO₂ nanoparticles at all concentrations and exposure times (P > 0.05). This study contributes to understanding biofilm formation and developing methods for controlling and effectively eliminating biofilms.

Özet (Çeviri)

Biofilms are a constant source of contamination on food contact surfaces in the food industry and play a role in transfer of pathogens, primarily Salmonella, posing serious health problems. In this study, biofilm formation of Salmonella serovars Enteritidis and Typhimurium on polystyrene microtiter plates, stainless steel (SS), and polypropylene (PP) coupons as well as eradication of preformed Salmonella biofilms using peracetic acid (PAA) and sodium hypochlorite (SH) disinfectants and silicon dioxide (SiO2) and titanium dioxide (TiO₂) nanoparticles was examined. All 21 Salmonella isolates produced biofilm on coupons. SH treatment with MIC and sub-MIC at 1 and 5 minutes on SS and PP coupons showed a ≥3 log reduction in biofilms of all isolates (3 to 6 log). The reduction in PAA on SS and PP coupons at MIC and ½ MIC for 1 and 5 minutes was between 0.3-5.2 log and 0.1-6.5 log, respectively, indicating statistical significance (P ≤ 0.001). SiO₂ (0.2-3.8 log) and TiO₂ (0.1-5.1 log) at tested concentrations and times on PP showed a decrease of 1 log or more (>90% reduction) in approximately half of the isolates. SiO₂ (0.1-2.1 log) and TiO₂ (0.1 3.6 log) with MIC and ½ MIC for 5 minutes on SS were more effective, showing a 1 log or more reduction (>90%) than for 1 minute. There was no statistically significant difference between results of SiO₂ and TiO₂ nanoparticles at all concentrations and exposure times (P > 0.05). This study contributes to understanding biofilm formation and developing methods for controlling and effectively eliminating biofilms.

Benzer Tezler

  1. Tez No

    203164

    Broylerlerde salmonella enteritidis ve salmonella typhimurium infeksiyonlarının ELISA ve drag swap yöntemleri ile incelenmesi

    The investigation of salmonella enteritidis and salmonella typhimurium infections using ELISA and drug swab methods

    SERKAN SÜLEYMAN ŞENGÜL

    Yüksek Lisans

    Türkçe

    Türkçe

    2008

    MikrobiyolojiAdnan Menderes Üniversitesi

    Veterinerlik Mikrobiyolojisi Ana Bilim Dalı

    YRD. DOÇ. DR. SÜHEYLA TÜRKYILMAZ

  2. Tez No

    881068

    Yeşil sentez yöntemiyle lacticaseibacillus rhamnosus ACS5 probiyotik bakterisi, prebiyotiği, postbiyotiği ile sentezlenen gümüş nanopartiküllerin antimikrobiyal ve antibiyofilm aktivitelerinin araştırılması

    Investigation of the antimicrobial and antibiofilm activities of silver nanoparticles synthesis with lacticaseibacillus rhamnosus ACS5 probiotic bacteria, prebiotics, postbiotics by the green synthesis method

    HİLAL ERDEN

    Yüksek Lisans

    Türkçe

    Türkçe

    2024

    BiyoteknolojiAksaray Üniversitesi

    Biyoteknoloji ve Moleküler Biyoloji Ana Bilim Dalı

    PROF. DR. DERYA ÖNAL DARILMAZ

  3. Tez No

    913885

    Hematopoetik kök hücre nakli yapılan hastalarda gelişen intestinal komplikasyonlar ile bağırsakta blastocystis spp. varlığı ilişkisinin araştırılması

    Investigation of the relationship between intestinal complications developing in patients who underwent hematopoietic stem cell transplantation and the presence of blastocystis spp. in the intestine

    BÜŞRA DEDECAN

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2024

    ParazitolojiAnkara Üniversitesi

    Tıbbi Mikrobiyoloji Ana Bilim Dalı

    PROF. DR. GÜLAY AKARSU

  4. Tez No

    70532

    Çocukluk yaş gruplarından izole edilen patojenlerin antibiyotik direnç paternlerinin NCCLS kriterlerine göre makrodilüsyon ve disk diffüzyon yöntemi kullanılarak araştırılması

    Investigation of antibiotic resistance patterns of enteric pathogens isolated from pediatric patients by macrodilution and disc diffusion methods according to NCCLS eritheria

    ÖZCAN ERDOĞAN

    Yüksek Lisans

    Türkçe

    Türkçe

    1998

    MikrobiyolojiGata

    Mikrobiyoloji ve Klinik Mikrobiyoloji Ana Bilim Dalı

    DOÇ. DR. TUNCER HAZNEDAROĞLU

  5. Tez No

    527471

    Yenidoğanlarda probiyotik desteğinin gastrointestinal sistemde kolonize vankomisine dirençli enterokok eradikasyonundaki rolünün araştırılması

    Investigation of the role of probiotic therapy in colonized vancomycin resistant enterococcus eradication in the gastrointestinal system in newborns

    MELEK BÜYÜKEREN

    Tıpta Yan Dal Uzmanlık

    Türkçe

    Türkçe

    2017

    Çocuk Sağlığı ve HastalıklarıHacettepe Üniversitesi

    Çocuk Sağlığı ve Hastalıkları Ana Bilim Dalı

    PROF. DR. ŞULE YİĞİT

Geri Dön