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Hastanede yatan hastaların klinik örneklerinden izole edilen Trichhosporon asahii kökenlerinde bazı virulans faktörlerinin araştırılması

Investigation of some virulence factors of Trichosporon asahii strains isolated from the clinical samples of hospitalized patients

  1. Tez No: 164079
  2. Yazar: AYLİN DAĞ
  3. Danışmanlar: DOÇ.DR. NİLGÜN ÇERİKÇİOĞLU
  4. Tez Türü: Yüksek Lisans
  5. Konular: Mikrobiyoloji, Microbiology
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2005
  8. Dil: Türkçe
  9. Üniversite: Marmara Üniversitesi
  10. Enstitü: Sağlık Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Mikrobiyoloji ve Klinik Mikrobiyoloji Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 73

Özet

2. SUMMARY Investigation of Some Virulence Factors of Trichosporon asahii Strains Isolated From the Clinical Samples of Hospitalized Patients In genus Trichosporon with relative resistance to antifungal therapy, T. asahii has emerged as a frequently isolated species from the clinical specimens of hospitalized patients in Turkish hospitals including Marmara University Hospital as well as in other parts of the world. In this study; acid proteinase, phospholipase, esterase activities, slime production and hydrophobic properties of a total of 48 T. asahii strains isolated from urine (43), periton (1), tongue (1), blood (1), nephrostomy (1) and nail (1) as a saprophite were investigated. In none of the 48 strains acid proteinase and phospholipase activities could be detected, while all were found to be esterase positive. In hydrocarbon assay performed with n-hexadecane 4 strains exhibited moderate hydrophobicity; while 43 strains showed low level hydrophobicity and 1 strain was found to be negative. When p-xylene was used as hydrocarbon source, 13 strains were found to be weakly hydrophobic; while remaining 35 strains did not exhibit surface hydrophobicity. In the second procedure to measure hydrophobicity by using microspheres assay, the results were found to be changing from strain to strain with the lowest and the highest values between 53.2 % and 92.7 %. Establishing the production of slime, modified tube assay was performed. Ten strains were found to be mild producers (++), 18 strains were weakly (+) and 20 strains were defined as non-slime producers. Our studty is to be the second in the literature in terms of investigation of acid proteinase and phospholipase activities, but the first for determining the existence of esterase activity, surface hydrophobicity and slime production in T. asahii strains. We may suggest that our results related to the presumptive virulence factors of T. asahii will contribute to the literature. However, to validate the clearcut relationships between thevirülansla ilişkilerinin net olarak belirlenebilmesi açısından klinik verilerle birlikte değerlendirilmeye gidilecek çalışmaların gerekli olduğu görüşündeyiz.

Özet (Çeviri)

2. SUMMARY Investigation of Some Virulence Factors of Trichosporon asahii Strains Isolated From the Clinical Samples of Hospitalized Patients In genus Trichosporon with relative resistance to antifungal therapy, T. asahii has emerged as a frequently isolated species from the clinical specimens of hospitalized patients in Turkish hospitals including Marmara University Hospital as well as in other parts of the world. In this study; acid proteinase, phospholipase, esterase activities, slime production and hydrophobic properties of a total of 48 T. asahii strains isolated from urine (43), periton (1), tongue (1), blood (1), nephrostomy (1) and nail (1) as a saprophite were investigated. In none of the 48 strains acid proteinase and phospholipase activities could be detected, while all were found to be esterase positive. In hydrocarbon assay performed with n-hexadecane 4 strains exhibited moderate hydrophobicity; while 43 strains showed low level hydrophobicity and 1 strain was found to be negative. When p-xylene was used as hydrocarbon source, 13 strains were found to be weakly hydrophobic; while remaining 35 strains did not exhibit surface hydrophobicity. In the second procedure to measure hydrophobicity by using microspheres assay, the results were found to be changing from strain to strain with the lowest and the highest values between 53.2 % and 92.7 %. Establishing the production of slime, modified tube assay was performed. Ten strains were found to be mild producers (++), 18 strains were weakly (+) and 20 strains were defined as non-slime producers. Our studty is to be the second in the literature in terms of investigation of acid proteinase and phospholipase activities, but the first for determining the existence of esterase activity, surface hydrophobicity and slime production in T. asahii strains. We may suggest that our results related to the presumptive virulence factors of T. asahii will contribute to the literature. However, to validate the clearcut relationships between thevirulence factors and pathogenesis of the infections, further studies including clinical evaluations must be carried out.

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