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Kan kültürlerinden izole edilen Staphylococcus aureus kökenlerinde sıvışık örtü (slime) üretiminin fenotipik ve genotipik olarak saptanması

Phenotypic and genotypic detection of slime production in Staphylococcus aureus strain isolated from blood cultures

  1. Tez No: 164078
  2. Yazar: ÖZGÜR ASLAN
  3. Danışmanlar: PROF.DR. FUNDA BABACAN
  4. Tez Türü: Yüksek Lisans
  5. Konular: Mikrobiyoloji, Microbiology
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2005
  8. Dil: Türkçe
  9. Üniversite: Marmara Üniversitesi
  10. Enstitü: Sağlık Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Mikrobiyoloji ve Klinik Mikrobiyoloji Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 59

Özet

not be able to amplify the genes of S. aureus. On the other hand as noted recently, another gene, Bap, may be responsible from the slime production in our isolates.

Özet (Çeviri)

2. SUMMARY Phenotypic and Genotypic Detection of Slime Production in Staphylococcus aureus Strain Isolated from Blood Cultures Slime production is one of the major factors in the adherence of bacterial cells to the host cells and biomaterials and causes significant increase in the invasion of bacteria. It has been shown that slime production is under the genetic control of ica operon in slime producing S. epidermidis strains and the activation of two genes (icaA and icaD), in this operon by the effects of environmental factors leads to increased amounts of slime production. Recent reports have also shown that these genes also exist in S. aureus strains and they play an important role in their slime production. In this study, we investigated the slime production of 100 blood culture isolates of S. aureus. Congo red agar (CRA) and several modifications of Tryptic soy broth (TSB) containing different amounts of glucose, EDTA and ferric citrate were used for phenotypic detection of slime production. For genotypic detection, PCR was performed and icaA and icaD genes were investigated by using two different primers for icaA and one primer for icaD. Phenotypically, iron restricted TSB was the only medium in which the slime production were mostly seen and 45 strains were found to be slime positive in this medium. icaD gene was detected in all strains. However, icaA gene was only found in 4 strains with“icaA 1 03 bp”primer. We could not detect icaA gene in all of the strains when PCR was performed with“icaA 1 88 bp”primer. In conclusion, iron restricted TSB medium seems to be a reliable method for the phenotypic detection of slime production in S. aureus. However, our genotypic results are not in agreement with both phenotyping results and with the litarature that state the activation of both icaA and icaD genes are required for slime production in S. aureus. Because the primers we used in the PCR test were specific for 5". Epidermidis, they maynot be able to amplify the genes of S. aureus. On the other hand as noted recently, another gene, Bap, may be responsible from the slime production in our isolates.

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