Antijenliği artırmaya yönelik yeni yaklaşımlar
Başlık çevirisi mevcut değil.
- Tez No: 355109
- Danışmanlar: PROF. DR. BEYAZIT ÇIRAKOĞLU
- Tez Türü: Doktora
- Konular: Tıbbi Biyoloji, Medical Biology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 1997
- Dil: Türkçe
- Üniversite: Marmara Üniversitesi
- Enstitü: Sağlık Bilimleri Enstitüsü
- Ana Bilim Dalı: Tıbbi Biyoloji Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 107
Özet
Özet yok.
Özet (Çeviri)
In studies of hybridoma cell and vaccine preparation, in order to obtain a specific antibody, the selection of adjuvants, especially those which may help to increase the antigenicity of a particular substance is essential. In this perspective ,in the study presented the use of polyelectrolyte adjuvants was investigated. For the development of a model system, Bovine serum albumine (BSA) was complexed with a number of different sytnthetic polymers which posses different physicochemical properties. Negatively charged polyacrilic acid (P AA) and BSA molecules were ligated by Cu2+ions and the light -scattering method stable complexes and conjugates, which were observed soluble or insoluble forms in water and their immunological properties were analysed. The results obtained from the analysis of the correlation between the physicochemical properties of polycomplexes and their immunological activities in vivo systems were as follows. (1) When polyanions were complex with BSA, the immuno response was increased by 4 to 5 fold, (2) this increase could only be observed only when the three substances, Cu2+, BSA and PAA were all participated in the complexes formed, and (3) it was found that antigens that were complexed with polymers required much shorter time of immunization than that of conventional immunization procedures. Based on the resuls obtained from the model system described above, the second part of the thesis was concerned with the immunological studies on BSA -Progesterone (BSAP) complexes and the complexes they formed with polymer adjuvants. When compared to the resuls of controls used, immuno responses specific to progesterone appeared to be considerably higher. A comparative study of immunologic properties of polycomplexes versus BSAP-mcomplete Freund's adjuvant (IF A) mixtures revealed specific differences in the antibody production. The immunized mice with BSAP+ polycomplexes and BSAP+ IF A were used for fusion. 12 monoclonal antibodies of IgG 1 and IgG2a types were obtained and the affinities of these antibodies were found to be 10.7_10.8 M . There was no cross reaction with the other steroid hormones. In consideration of the whole data obtained, it can be suggested that a helpful method for the increase of the immunogenicity of antigens with little or no immunogenicity was described.
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