Antijenliği artırmaya yönelik yeni yaklaşımlar
Başlık çevirisi mevcut değil.
- Tez No: 355109
- Danışmanlar: PROF. DR. BEYAZIT ÇIRAKOĞLU
- Tez Türü: Doktora
- Konular: Tıbbi Biyoloji, Medical Biology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 1997
- Dil: Türkçe
- Üniversite: Marmara Üniversitesi
- Enstitü: Sağlık Bilimleri Enstitüsü
- Ana Bilim Dalı: Tıbbi Biyoloji Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 107
Özet
Özet yok.
Özet (Çeviri)
In studies of hybridoma cell and vaccine preparation, in order to obtain a specific antibody, the selection of adjuvants, especially those which may help to increase the antigenicity of a particular substance is essential. In this perspective ,in the study presented the use of polyelectrolyte adjuvants was investigated. For the development of a model system, Bovine serum albumine (BSA) was complexed with a number of different sytnthetic polymers which posses different physicochemical properties. Negatively charged polyacrilic acid (P AA) and BSA molecules were ligated by Cu2+ions and the light -scattering method stable complexes and conjugates, which were observed soluble or insoluble forms in water and their immunological properties were analysed. The results obtained from the analysis of the correlation between the physicochemical properties of polycomplexes and their immunological activities in vivo systems were as follows. (1) When polyanions were complex with BSA, the immuno response was increased by 4 to 5 fold, (2) this increase could only be observed only when the three substances, Cu2+, BSA and PAA were all participated in the complexes formed, and (3) it was found that antigens that were complexed with polymers required much shorter time of immunization than that of conventional immunization procedures. Based on the resuls obtained from the model system described above, the second part of the thesis was concerned with the immunological studies on BSA -Progesterone (BSAP) complexes and the complexes they formed with polymer adjuvants. When compared to the resuls of controls used, immuno responses specific to progesterone appeared to be considerably higher. A comparative study of immunologic properties of polycomplexes versus BSAP-mcomplete Freund's adjuvant (IF A) mixtures revealed specific differences in the antibody production. The immunized mice with BSAP+ polycomplexes and BSAP+ IF A were used for fusion. 12 monoclonal antibodies of IgG 1 and IgG2a types were obtained and the affinities of these antibodies were found to be 10.7_10.8 M . There was no cross reaction with the other steroid hormones. In consideration of the whole data obtained, it can be suggested that a helpful method for the increase of the immunogenicity of antigens with little or no immunogenicity was described.
Benzer Tezler
- Targeting and activation of antigen specific CD8+ T cells with peptide-major histocompatibility complex I tetramers
Peptit-majör histokompatibilite kompleks I tetramerleri ile antijen spesifik CD8+ T hücrelerin hedeflenmesi ve aktivasyonu
ŞAFAK CEREN USLU ŞIVGIN
Doktora
İngilizce
2025
BiyoteknolojiHacettepe ÜniversitesiTemel Onkoloji Ana Bilim Dalı
DOÇ. DR. HANDE CANPINAR
- Hepatit B aşısı geliştirme ve hepatit B aşılarının etkinliğini artırmaya yönelik yaklaşımlar
Approaches for the development of HBV vaccine and improvement the effectiveness of HBV vaccine
İBRAHİM HATİPOĞLU
Doktora
Türkçe
2012
BiyolojiAnadolu ÜniversitesiBiyoloji Ana Bilim Dalı
DOÇ. DR. AYNUR BAŞALP
PROF. DR. HÜLYA SİVAS
- Küçük hücreli dışı akciğer kanserine karşı yeni bir peptit aşının in silico tasarımı
In silico design of a novel peptide vaccine to combat non-small cell lung cancer
SEYEDEHNAVA AHRAR
Yüksek Lisans
Türkçe
2025
OnkolojiEge ÜniversitesiTemel Onkoloji Ana Bilim Dalı
PROF. DR. RALPH LEO JOHAN MEUWISSEN
- Canine parvovirus ve canine coronavirus etkenlerin eş zamanlı teşhisine yönelik kit geliştirilmesi
Development of a diagnostic kit for the simultaneous diagnosis of canine parvovirus and canine coronavirus agents
ÇAĞRI AVCİ
Doktora
Türkçe
2024
Veteriner HekimliğiSelçuk ÜniversitesiVeterinerlik Viroloji Ana Bilim Dalı
PROF. DR. SİBEL YAVRU
- Kedilerde felıne ımmunodefıcıency virus (FIV) ve felıne leukaemıa vırus (FELV) enfeksiyonlarının pcr ve ELISA ile saptanarak herpes virüs ve diğer risk faktörlerinin araştırılması.
Detection of feline immunodeficiency virus (FIV) and feline leukaemia virus (FELV) infections in cats by pcr and ELISA and investigation of herpes virus and other the risk factors .
ERHAN BAYRAKTAR
Doktora
Türkçe
2006
Mikrobiyolojiİstanbul ÜniversitesiMikrobiyoloji (Veterinerlik) Ana Bilim Dalı
PROF.DR. HÜSEYİN YILMAZ