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Biotin-Streptavidin modelinde immünojenik olmayan yapılara karşı antikor üretimi ve molekülsel temellerinin irdelenmesi

Başlık çevirisi mevcut değil.

  1. Tez No: 355212
  2. Yazar: MUZAFFER TAYLAN
  3. Danışmanlar: DR. EROL ÖMER ATALAY
  4. Tez Türü: Yüksek Lisans
  5. Konular: Genetik, Tıbbi Biyoloji, Genetics, Medical Biology
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 1998
  8. Dil: Türkçe
  9. Üniversite: Marmara Üniversitesi
  10. Enstitü: Sağlık Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Tıbbi Biyoloji ve Genetik Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 117

Özet

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Özet (Çeviri)

In our day, industrial and scientific applications based on the concept of recognition among molecules have a wide-spread usage in fields like medicine, veterinary sciences and agriculture. The developments which have, in recent years, taken place in molecular biology have been reflected on various disciplines and as a natural result of this, there has been an increase in the requirements in these fields. These increases in requirements have resulted in a number of problems pertaining to the approaches used and the application of the technology in these approaches. In cases where technology is not adequate, the possibilities created by the developing recombinant DNA technology contribute to overcoming the problems. The Phage-display technology developed along these lines has its place among the current approaches reached by the developments of the hybridoma technology in these applications. When one thinks within the scope of epitope-paratope relations, it can be seen that a molecule with a recognition function does not necessarily have to be very large. When Phage-display technology is used it is possible to have more detailed studies wit these recognition points. Starting to use of peptide libraries artificially prepared to imitate these recognition points in choosing structures which conformationally recognize a certain target, facilitates the production of more original structures, helps in alliviating the usage of live animals and thus promotes the solving of various problems stemming from the immune system functions of the organism used. In this respect, the immunogenecity specifications foreseen by classical methods can be overlooked in the chosen target molecule. In this thesis, in order to produce molecules which can conformationally recognize non-immunogenic structures in the biotin-streptavidin model, streptavidin which does not have an immunogenic effect has been designated as the nonimmunogenic target molecule and the polypeptide sequence which can recognize it has been selected from an artificially produced 16-mer long peptide library through the usage of phage-display technology. It has been shown that the selected polypeptide is active even when it is independent from filamentous bacteriophage, and by DNA sequence analysis, it has compared to those of previous studies and the similarities among these results have been studied.

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