Axonal regeneration and expression of neuropeptides and neurofilaments in primary sensory neurons in vitro
Başlık çevirisi mevcut değil.
- Tez No: 400195
- Danışmanlar: DR. DAVİD TONGE
- Tez Türü: Doktora
- Konular: Psikoloji, Psychology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 1999
- Dil: İngilizce
- Üniversite: University of London
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 177
Özet
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Özet (Çeviri)
Following a peripheral nerve injury, a number of changes occur in thephenotype of primary sensory neurons of dorsal root ganglia (DRGs). Thesechanges are believed to reflect a switch in metabolic activity of neurons fromsignal transmission to repair and regeneration. The mechanisms regulating theseevents are not fully known. The deprivation of neurons of certain trophic factorsand synthesis or upregulation of others are possible explanations for thesechanges. In this thesis, explant cultures of adult mouse DRGs were used toinvestigate the effects of some of these factors on neuronal survival, axonalgrowth, and neuropeptide and neurofilament (NF) expression in primary sensoryneurons and in regenerating axons using immunohistochemical techniques.The proportion of surviving neurons was very high in cultured DRGsduring 3-day incubation. Nerve growth factor (NGF) appeared to supportneuronal survival and strongly stimulated growth of axons. LIF did not affectoverall survival rate of these neurons and did not enhance axonal growth.However it increased the diameter of growing axons under certain conditionsand also strongly upregulated galanin (GAL) expression in neurons and growingaxons which was partially antagonized by NGF administration.In adult animals, NFs play a major role in determining axonal diameterand conduction velocity, but little is known about the mechanisms by which NFexpression is regulated. In this study, I investigated expressions of NF subunitsin regenerating axons using immunohistochemistry and observed that NF-Hwas expressed before NF-M and NF-L. The neurotrophins NGF and brainderivedneurotrophic factor (BDNF) accelerated NF expression, butneurotrophin-3 (NT-3) was ineffective. However, axonal contact with the distalstump of a nerve in culture was far more effective than the neurotrophins inpromoting NF expression.Finally, the effects of diffusible factors from lesioned nerves on axonalgrowth were investigated and shown to be mainly due to NGF, which would beexpected to promote regeneration of only those axons expressing the appropriateNGF receptor, trkA. The effects of FGF-1 were also investigated and evidencepresented that this factor may be able to stimulate regeneration of axons otherthan those expressing trkA.
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