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Molecular marker assisted analysis of grain yield and related traits in barley

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  1. Tez No: 400544
  2. Yazar: NEJDET KANDEMİR
  3. Danışmanlar: PROF. DR. ANDRIS KLEINHOFS
  4. Tez Türü: Doktora
  5. Konular: Botanik, Ziraat, Botany, Agriculture
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 1999
  8. Dil: İngilizce
  9. Üniversite: Washington State University
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Toprak Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 114

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Özet (Çeviri)

Mapping of quantitative traits is a very active topic in biology. Quantitative trait loci (QTL) data have to be verified and validated for usefulness in plant breeding. The present study aims to determine the usefulness of previously identified yield QTL for barley (Hordeum vulgare L.) breeding. Three previously mapped grain yield QTL on chromosomes 2, 3 and 5 were studied in Steptoe x Morex cross using near isogenic lines (NILs). The chromosome 2 yield QTL region of Mofex delayed flowering time of Steptoe by 11 days but did not affect grain yield. The Steptoe chromosome 3 yield QTL, which is the largest yield QTL in Steptoe x Morex cross, reduced lodging and head shattering when introduced into the moderate yielding, high malting quality barley, Morex. Grain yield of Morex, however, was not improved. The chromosome 5 yield QTL transferred from Steptoe to Morex caused slight yield increase in all NILs although differences were not significant. Two major and a minor QTL were detected on chromosomes 2,3 and 7 for head shattering. The largest QTL on chromosome 3 was located in a 0.6 cM region using NILs. We detected two flowering time QTL with photoperiod response (Phr) on chromosomes 2S and 5L (designated Phr-2Sand Phr-SL) in positions similar to the previously mapped PpdHI and PpdH2 loci, respectively. NIL analyses located Phr-2S andPhr-5L in 0.9 and 6.0 cM intervals. Results suggested the presence of additional loci for flowering time. NILs developed in this study will be useful to trace additional loci for the respective traits using QTL mapping. The QTL introgressed into the NILs in this study were the major ones. The major QTL make detection of relatively small effect QTL difficult. Minor QTL can be more readily detected if studied in crosses where the major QTL are fixed. Minor QTL could be useful in plant breeding. Discrete phenotype classes created in NILs will be useful in fine mapping for map based cloning of QTL.

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