Using bulked segregant analysis to detect SSR marker(s) linked to the Phytophthora resistance loci of soybean
Başlık çevirisi mevcut değil.
- Tez No: 401102
- Danışmanlar: PROF. JAMES E. SPECHT
- Tez Türü: Yüksek Lisans
- Konular: Ziraat, Agriculture
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 1998
- Dil: İngilizce
- Üniversite: University of Nebraska-Lincoln
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 91
Özet
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Özet (Çeviri)
Phytophthora root rot disease of soybean, caused by Phytophthora megasperma Drechs. f. sp. glycinea Kuan and Ervin, can cause substantial economic losses in soybeans. Seven major dominant genes with alleles giving race-specific resistance to phytophthora have been reported in soybean. The objective of this study was to identify linkage between simple sequence repeat (SSR) markers and the six Rps loci {Rps1 to Rps6),using a bulked segregant analysis technique, a recently developed SSR map of soybean, and the phenotyped progeny derived from the mating of a near-isogenic line of a susceptible {rps) near-isogenic line (NIL) of the cultivar Harosoy (i.e., Harosoy- E2) with a series of 'Clark' near-isogenic lines, each of the latter possessing a different phytophthora resistance {Rps) allele. Mapped SSR markers were screened for polymorphism between the Clark NILs and the Harosoy NIL. The percentage of SSR markers assayed that exhibited parental polymorphism ranged from 11.4% to 30.7% averaging about 27.4%over all matings. The parents (Clark and Harosoy) plus the two bulks (homozygous dominant and resistant) were then assayed with the polymorphic SSR ioci to determine if putative genetic linkage existed between the Rps loci and the polymorphic SSR marker loci. Markers found to be polymorphic between bulks were considered putatively linked to the Rps locus in question. In those cases, F2 homozygous individuals (both recessive and dominant) were assayed to confirm or refute the putative linkages. Analyses of data revealed linkage of one or more SSR markers to each of the six phytophthora loci. The strongest linkages were Sattl 52 with Rps1 (p=0.133±0.072), Rpslb (p=0.164±0.069), and Rpslc (p=0.237±0.080), Sattl 59 with Rpslk (p=0.164±0.071), Satt547 with Rps2 (p=0.238±0.093), HSP176 with Rps3 (p=0.245±0.085), Satt472 with Rps4 (p= 0.083±0.056), Sattl99 with Rps5 (p=0.331 ±0.081), and Sattl91 with Rps6 (p=0.216±0.081). No SSR was found to be linked to Rpsld or Rps3c, suggesting that the phytophthora resistance segregating in these two populations was not at the indicated locus.
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