Genetic analysis of stoned B/stonin 2 function in vivo
Başlık çevirisi mevcut değil.
- Tez No: 401192
- Danışmanlar: PROF. DR. VOLKER HAUCKE
- Tez Türü: Doktora
- Konular: Tıbbi Biyoloji, Medical Biology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2005
- Dil: İngilizce
- Üniversite: Georg-August-Universität Göttingen
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 144
Özet
Özet yok.
Özet (Çeviri)
Clathrin-mediated endocytosis at presynaptic nerve terminals is a regulated process tightly coupled to the exocytotic fusion event that requires clathrin, dynamin, AP2 as well as synaptotagmin 1. Stonin 2 is a brain-enriched protein which localizes to axonal vesicle clusters in neurons and clathin/AP2-coated pits in astroglial cells. Its Drosophila orthologue stoned B has a putative role in synaptic vesicle recycling, especially sorting and endocytosis of synaptotagmins. Mammalian stonin 2 interacts with the heterotetrameric clathrin adaptor AP2, via three novel WVXF motifs, and with other endocytic accessory proteins eps15 and intersectin 1 by virtue of its two NPF motifs in its amino-terminal domain. The carboxy-terminal μHD binds to synaptotagmin 1 and is required for proper localization of stonin 2 to synapses in neurons and to coated-pits in astroglial cells. We have recently developed an in vivo interaction assay, to test the interactions between stonins and synaptotagmin family members (1-11). Predominantly cytosolic stonin 2, translocates to the plasmalemma and colocalizes with AP2-coated pits when co-expressed together with synaptotagmins 1, 2, and 9. Transgenic expression of stonin 2 in a stable HEK293 cell line expressing lumenally FLAG-tagged synaptotagmin 1, stimulates endocytic internalization of plasma membrane-stranded synaptotagmin 1 more than 20-fold. Stonin 2 thus physically links synaptotagmin 1 and AP2 and this linkage is required for synaptotagmin 1 endocytosis. Disruption of the stonin 2-AP2 interaction by mutagenesis of WVXF motifs or RNAi-mediated silencing of AP2 expression, inhibits stonin 2-assisted synaptotagmin 1 uptake. In neuroendocrine PC12 cells, stonin 2 assists in targeting of internalized synaptotagmin 1 to SLMVs in addition to facilitating its endocytosis. For a better characterization of its functions, a stonin 2-deficient mouse strain was created by targeted disruption of the stonin 2 gene. Stonin 2 knock-out animals are viable and fertile. Preliminary analysis of synaptotagmin 1 distribution, endocytic recycling, number and morphology of synaptic vesicles in stonin 2 deficient nerve terminals did not display any abnormalities compared to wildtype littermates. Therefore refined experimental procedures seem to be required for characterization of stonin 2 deficiency in neurons and astrocytes. We propose that stonin 2 is an endocytic sorting adaptor for clathrin-mediated endocytosis of synaptotagmins in neurons and neurosecretory cells, as well as yet unidentified cargo molecule(s) in astroglial cells.
Benzer Tezler
- Probiyotiklerin un biti, Tribolium castaneum (Herbst, 1797)'un bazı biyolojik özellikleri üzerine etkisi
Effects of probiotics to some biological properties of Tribolium castaneum Herbst 1797 (Coleoptera: Tenebrionidae)
YILMAZ ŞAHİN
Yüksek Lisans
Türkçe
2019
BiyolojiErciyes ÜniversitesiBiyoloji Ana Bilim Dalı
PROF. DR. AYDIN ŞÜZÜ TUNÇBİLEK
- Jeotermal ve güneş enerjisinin elektrik ve hidrojen üretimi, ısıtma ve soğutma için kullanılmasının yapay sinir ağları ile modellenmesi, termoekonomik analizi ve optimizasyonu
Artificial neural networks modeling and thermoeconomic analysis and optimization of geothermal and solar energy use in electricity and hydrogen generation, heating and cooling
OZAN ŞEN
Yüksek Lisans
Türkçe
2021
Makine MühendisliğiAfyon Kocatepe ÜniversitesiMakine Mühendisliği Ana Bilim Dalı
DOÇ. CEYHUN YILMAZ
- Isolation and characterization of Bacillus thuringiensis strains from different grain habitats
Farklı tahıl ortamlarından Bacillus thuringiensis izolasyonu ve karakterizasyonu
ÖZGÜR APAYDIN
Yüksek Lisans
İngilizce
2004
Biyomühendislikİzmir Yüksek Teknoloji EnstitüsüBiyoteknoloji Ana Bilim Dalı
DOÇ.DR. HATİCE GÜNEŞ
- Music retrieval systems: Robust performance under the effect of uncertainty
Başlık çevirisi yok
ERDEM ÜNAL
Doktora
İngilizce
2008
Elektrik ve Elektronik MühendisliğiUniversity of Southern CaliforniaDR. SHRIKANTH NARAYANAN
- Mekansal analiz teknikleri ile çok kriterli karar verme yaklaşımı kullanılarak raylı sistem güzergah analizi
Rail system route analysis using multi criteria decision making with spatial analysis techniques
BERNA ÇALIŞKAN
Doktora
Türkçe
2023
Ulaşımİstanbul Teknik Üniversitesiİnşaat Mühendisliği Ana Bilim Dalı
PROF. DR. ALİ OSMAN ATAHAN