Genetic analysis of stoned B/stonin 2 function in vivo
Başlık çevirisi mevcut değil.
- Tez No: 401192
- Danışmanlar: PROF. DR. VOLKER HAUCKE
- Tez Türü: Doktora
- Konular: Tıbbi Biyoloji, Medical Biology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2005
- Dil: İngilizce
- Üniversite: Georg-August-Unıversıtat Gottıngen
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: Belirtilmemiş.
Özet
Özet yok.
Özet (Çeviri)
Clathrin-mediated endocytosis at presynaptic nerve terminals is a regulated process tightly coupled to the exocytotic fusion event that requires clathrin, dynamin, AP2 as well as synaptotagmin 1. Stonin 2 is a brain-enriched protein which localizes to axonal vesicle clusters in neurons and clathin/AP2-coated pits in astroglial cells. Its Drosophila orthologue stoned B has a putative role in synaptic vesicle recycling, especially sorting and endocytosis of synaptotagmins. Mammalian stonin 2 interacts with the heterotetrameric clathrin adaptor AP2, via three novel WVXF motifs, and with other endocytic accessory proteins eps15 and intersectin 1 by virtue of its two NPF motifs in its amino-terminal domain. The carboxy-terminal μHD binds to synaptotagmin 1 and is required for proper localization of stonin 2 to synapses in neurons and to coated-pits in astroglial cells. We have recently developed an in vivo interaction assay, to test the interactions between stonins and synaptotagmin family members (1-11). Predominantly cytosolic stonin 2, translocates to the plasmalemma and colocalizes with AP2-coated pits when co-expressed together with synaptotagmins 1, 2, and 9. Transgenic expression of stonin 2 in a stable HEK293 cell line expressing lumenally FLAG-tagged synaptotagmin 1, stimulates endocytic internalization of plasma membrane-stranded synaptotagmin 1 more than 20-fold. Stonin 2 thus physically links synaptotagmin 1 and AP2 and this linkage is required for synaptotagmin 1 endocytosis. Disruption of the stonin 2-AP2 interaction by mutagenesis of WVXF motifs or RNAi-mediated silencing of AP2 expression, inhibits stonin 2-assisted synaptotagmin 1 uptake. In neuroendocrine PC12 cells, stonin 2 assists in targeting of internalized synaptotagmin 1 to SLMVs in addition to facilitating its endocytosis. For a better characterization of its functions, a stonin 2-deficient mouse strain was created by targeted disruption of the stonin 2 gene. Stonin 2 knock-out animals are viable and fertile. Preliminary analysis of synaptotagmin 1 distribution, endocytic recycling, number and morphology of synaptic vesicles in stonin 2 deficient nerve terminals did not display any abnormalities compared to wildtype littermates. Therefore refined experimental procedures seem to be required for characterization of stonin 2 deficiency in neurons and astrocytes. We propose that stonin 2 is an endocytic sorting adaptor for clathrin-mediated endocytosis of synaptotagmins in neurons and neurosecretory cells, as well as yet unidentified cargo molecule(s) in astroglial cells.
Benzer Tezler
- Ferrokrom fabrikasında çalışan işçilerde stogenetik çalışmalar
Başlık çevirisi yok
AYNUR ACAR
Doktora
Türkçe
1985
Tıbbi BiyolojiAkdeniz ÜniversitesiTıbbi Biyoloji ve Genetik Ana Bilim Dalı
DOÇ. DR. GÜVEN LÜLECİ
- Bazı introdüksiyon patates populasyonlarında; verim ve verimle ilgili ögeler arasındaki ilişkiler
Başlık çevirisi yok
SEFAİ GENÇ
Yüksek Lisans
Türkçe
1987
ZiraatEge ÜniversitesiTarla Bitkileri Ana Bilim Dalı
PROF. DR. M. BİRKAN YILDIRIM
- Patateste farklı fizyolojik yaş dönemlerinin verim ve kalite özellikleri üzerine etkileri
Başlık çevirisi yok
F.SEVDA BİGALI
Yüksek Lisans
Türkçe
1986
ZiraatEge ÜniversitesiBitki Islahı ve Genetik Ana Bilim Dalı
PROF. DR. METİN BİRKAN YILDIRIM
- Sivas yöresinde akraba evliliği yapan ve yapmayan çiftlerde dermatoglifik benzerlikler
Similarities of dermataoglyphies among individval of married relatives and married couples not related to each other in Sivas region
Y. SELMA SÜNGÜ
Yüksek Lisans
Türkçe
1984
Tıbbi BiyolojiCumhuriyet ÜniversitesiTıbbi Biyoloji ve Genetik Ana Bilim Dalı
YRD. DOÇ. DR. İLHAN SEZGİN