Just how good is third generation sequencing for complex genomes?
Başlık çevirisi mevcut değil.
- Tez No: 401420
- Danışmanlar: DR. EDWARD HOLLOX
- Tez Türü: Yüksek Lisans
- Konular: Genetik, Genetics
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2013
- Dil: İngilizce
- Üniversite: Unıversıty Of Leıcester
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: Belirtilmemiş.
Özet
Özet yok.
Özet (Çeviri)
The sequencing technologies have been pioneers in field of genomics. Sanger sequencing as a first generation sequencing (FGS) technique has been the first significant method for Human Genome Project. Numbers of publishes had done through this method but it had to be improved in terms of read length, time consuming and high cost. To reduce cost and increase throughput, second generation sequencing (SGS) techniques have appeared on market. However, still read length has been the main challenge for complex genome sequencing. In this case, third generation sequencing techniques (TGS) have provided large-scale sequencing and real time single molecule detection to exceed previous limitations. Our study covers of testing Pacific Bioscience Single Molecule Real Time Sequencing Technique (SMRT) as a TGS method to analyse both the single base and the molecular level of complex genomes, also assemble a contiguous BAC sequence across the macaque beta-defensin region, and perform a preliminary analysis of sequence variation. Analyses of restriction enzyme maps and BAC end sequencing have referred misassembling and also data analysis of gene content has confirmed sequence error in TGS. As a result, this study has demonstrated that SMRT technology has some gaps for sequence and assembling accuracy even though it provides long read length and direct assembly. Therefore, in future, enzyme kinetics can be improved to increase the accuracy of single molecule sequencing in real time. Furthermore, if higher level dimensional data is accurately analysed and assimilated, TGS will contribute towards a better understanding of large-scale DNA sequencing.
Benzer Tezler
- Tarihi ve kültürel sürekliliğin fiziksel çevrede değişime yansıması ve Eskişehir örneği
The Reflections of historical and cultural continuity on the changes of physical environment and the Eskişehir example
MERAL NALÇAKAN
- A tipi yatırım fonlarında etkin bir nakit yönetim stratejisi oluşturmada analitik hiyerarşi yaklaşımı
Başlık çevirisi yok
FERAL YOLAL
- Çok boyutlu kaotik sistemler ile şifreleme
Encryption with multi-dimensional chaotic systems
ASİYE YİĞİT
Yüksek Lisans
Türkçe
1997
İstanbul Teknik ÜniversitesiElektronik ve Haberleşme Mühendisliği Ana Bilim Dalı
Yükleniyor...