Geri Dön

The modification of carbon electrodes for biosensor applications

Başlık çevirisi mevcut değil.

PDF İndir

  1. Tez No: 401579
  2. Yazar: İZZET KOÇAK
  3. Danışmanlar: DR. GUY DENUAULT, DR. CHRIS SKYLARIS
  4. Tez Türü: Doktora
  5. Konular: Kimya, Chemistry
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2013
  8. Dil: İngilizce
  9. Üniversite: University of Southampton
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 202

Özet

Özet yok.

Özet (Çeviri)

This thesis reports upon the covalent attachment of 6 different types of linkers bearing the Boc protecting group to the surface of glassy carbon electrodes, highly ordered pyrolytic graphite with the edge and basal plane orientation and multi-walled carbon nanotube abrasively immobilised onto a glassy carbon substrate by electrochemical oxidation or reduction of the corresponding diazonium salt. Removal of the Boc group allows redox probes, such as anthraquinone-2-carboxyl acid, 4 nitrobenzoyl chloride and 3 and 4 dihydroxybenzoyl chloride, to be coupled to the surface using solid-phase coupling methods. The surface coverage, scan rate and pH effects, as well as the determination of the kinetic parameters, were evaluated using cyclic voltammetry. It was found that the type of carbon electrode and the choice of linkers had a significant influence on the surface coverage of redox probes and the electron transfer rate. A 4-(N-Boc-aminomethyl) benzene diazonium tetrafluoroborate salt linker (C6H4CH2NH) was also spontaneously grafted onto the CNTs by refluxing in the C6H4CH2NHBoc diazonium salt at 60 oC in an acetonitrile solution. After the removal of the Boc protecting group, the anthraquinone (AQ) and nitrobenzene (NB) groups were attached to the benzyl amine linker by solid-phase amide coupling. The grafted CNTs were characterized using FTIR and cyclic voltammetry techniques; the surface coverage and the stability of the tethered functional groups were also investigated. The oxygen reduction reaction was studied on bare and anthraquinone (AQ) modified edge planes, on a basal plane with highly oriented pyrolytic graphite, and on glassy carbon (GC) and multi-walled carbon nanotube electrodes. The anthraquinone-modified rotating disc GC electrode results show the two electron oxygen reduction reactions with hydrogen peroxide as the final product. The immobilization of laccase (ThL) onto GC electrodes modified with anthraquinone and anthracene through EDA and C6H4CH2NH– linkers was also achieved by employing high-throughput screening using a multichannel potentiostat for a library of 12 electrodes; the activity of these electrodes to oxygen reduction was examined. The experimental findings demonstrated a successful attachment of laccase to the modified glassy carbon, as well as successful electron transfer between substrate- and enzyme-active sites. DFT calculations were used to investigate the structural properties of the functionalized basal plane of graphene after modification and to discover why the edge site shows higher reactivity to the attachment of linkers than the basal sites and whether the type of linker has an effect on the surface coverage of AQ. On the basis of considering the relationship between binding energy and charge transfer, the root of the effect of the type of linker on surface coverage was investigated to ensure whether it is steric, electronic, or both.

Benzer Tezler

  1. Tez No

    368779

    Development of novel immobilization matrices via surface modification approach for glucose detection and their biosensor applications

    Glukoz tayini için yeni immobilizasyon matrislerinin yüzey modifikasyonu yaklaşımı ile geliştirilmesi ve biyosensör uygulamaları

    SEMA DEMİRCİ UZUN

    Doktora

    İngilizce

    İngilizce

    2014

    KimyaOrta Doğu Teknik Üniversitesi

    Polimer Bilim ve Teknolojisi Ana Bilim Dalı

    PROF. DR. LEVENT KAMİL TOPPARE

    PROF. DR. SUNA TİMUR

  2. Tez No

    498507

    Nanocomposites with graphene

    Grafen esaslı nanokompozitler

    SİBEL DEMİROĞLU

    Doktora

    İngilizce

    İngilizce

    2018

    Bilim ve Teknolojiİzmir Katip Çelebi Üniversitesi

    Nanobilim ve Nanoteknoloji Ana Bilim Dalı

    DOÇ. DR. MEHMET ÖZGÜR SEYDİBEYOĞLU

  3. Tez No

    223272

    Electropolymerization of N-pyrrole, N -phenylpyrrole, 1-(4-methoxyphenyl)-1H-pyrrole on carbon fiber micro electrodes, characterizations and biosensor applications

    N-pirol, N-fenilpirol ve 1-(4-metoksifenil)-1H-pirol’ün karbon fiber mikro elektrot üzerine elektropolimerizasyonu, karakterizasyonu ve biyosensör uygulamaları

    SİBEL SEZGİN

    Yüksek Lisans

    İngilizce

    İngilizce

    2007

    Polimer Bilim ve Teknolojisiİstanbul Teknik Üniversitesi

    Polimer Bilim ve Teknolojisi Ana Bilim Dalı

    PROF.DR. A. SEZAİ SARAÇ

  4. Tez No

    649961

    Karbon nanotüp temelli kompozitlere dayalı elektrokimyasal biyosensörlerin geliştirilmesi ve miRNA tayinine yönelik uygulamaları

    Development of electrochemical biosensors based on carbon nanotube based composites and their applications on the detection of miRNA

    SELEN SOYALP

    Yüksek Lisans

    Türkçe

    Türkçe

    2020

    KimyaEge Üniversitesi

    Malzeme Bilimi ve Mühendisliği Ana Bilim Dalı

    PROF. DR. KADRİYE ARZUM ERDEM GÜRSAN

  5. Tez No

    467190

    Synthesis of some functional conducting polymers, characterization, Mott-Schottky analysis and sensor applications

    Bazı fonksiyonel iletken polimerlerin sentezi, karakterizasyonu, Mott-Schottky analizi ve sensor uygulamaları

    TOLGA KARAZEHİR

    Doktora

    İngilizce

    İngilizce

    2017

    Polimer Bilim ve Teknolojisiİstanbul Teknik Üniversitesi

    Polimer Bilim ve Teknolojisi Ana Bilim Dalı

    PROF. DR. ABDÜLKADİR SEZAİ SARAÇ

    PROF. DR. MURAT ATEŞ

Geri Dön