Geri Dön

Allojenik ve alloplastik greft materyallerinin periodontal ligament fibroblastları ve MC3T3-E1 hücrelerinin biyolojik aktivitelerine olan etkilerinin in vitro incelenmesi

Assesment an in vitro experimental system biological effects of the allogenics and alloplastic graft materials on the periodontal ligament fibroblasts and MC3T3-E1 cells

  1. Tez No: 48682
  2. Yazar: K. DİDEM DEVECİOĞLU
  3. Danışmanlar: PROF.DR. DİLEK ŞENGÜN
  4. Tez Türü: Doktora
  5. Konular: Diş Hekimliği, Dentistry
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 1996
  8. Dil: Türkçe
  9. Üniversite: Hacettepe Üniversitesi
  10. Enstitü: Sağlık Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 87

Özet

SUMMARY The ultimate goal of traditional periodontal therapy is to resolve the inflammatory lesions in the periodontal tissues. For many decades, researchers have been interested in promoting regenaration of tooth- supporting structures destroyed by inflammatory periodontal diseases. This requires regeneration of fibrous connective tissue, formation of new bone, and reattachment of new connective tissue fibers into newly formed cementum. When appropriate! ly triggered, periodontal ligament cells are thought to play an important role in promoting periodontal regeneration. Thus considerable research efforts have focused on PDL cells to promote migration and proliferation to root surface and synthesis of new fibrous tissue. For this purpose, several flap tecniques, bone grafts, barter membranes and biochemical agents are used for periodontal regeneration. In this study we have focused on establishing biological activities of allografts (DDKD, CKA and cementum) and alloplastic graft material (Biocoral) in an in vitro experimental system. The biological activities of DDKD, CKA, cementum and Biocoral examined included their ability to promote proliferation attachment and protein content of human PDL fibroblasts and MC3T3-E1 cells, isolated from mouse osteosarcoma. None of the grafting materials didn't have any inhibitory effect on both of cell groups. Biocoral elicited a statistically significiant increase in total protein production compared to controls on the PDL fibroblasts on 4th day. In 70rapid attachment assay, effects of CKA and Biocoral have been found statitically significant compared to other grafting materials and an enhanced attachment of surface unit have been detected in Biocoral group. The highest number of mineral like nodules have been observed in DDKD group on MC3T3-E1 cells. 71

Özet (Çeviri)

SUMMARY The ultimate goal of traditional periodontal therapy is to resolve the inflammatory lesions in the periodontal tissues. For many decades, researchers have been interested in promoting regenaration of tooth- supporting structures destroyed by inflammatory periodontal diseases. This requires regeneration of fibrous connective tissue, formation of new bone, and reattachment of new connective tissue fibers into newly formed cementum. When appropriate! ly triggered, periodontal ligament cells are thought to play an important role in promoting periodontal regeneration. Thus considerable research efforts have focused on PDL cells to promote migration and proliferation to root surface and synthesis of new fibrous tissue. For this purpose, several flap tecniques, bone grafts, barter membranes and biochemical agents are used for periodontal regeneration. In this study we have focused on establishing biological activities of allografts (DDKD, CKA and cementum) and alloplastic graft material (Biocoral) in an in vitro experimental system. The biological activities of DDKD, CKA, cementum and Biocoral examined included their ability to promote proliferation attachment and protein content of human PDL fibroblasts and MC3T3-E1 cells, isolated from mouse osteosarcoma. None of the grafting materials didn't have any inhibitory effect on both of cell groups. Biocoral elicited a statistically significiant increase in total protein production compared to controls on the PDL fibroblasts on 4th day. In 70rapid attachment assay, effects of CKA and Biocoral have been found statitically significant compared to other grafting materials and an enhanced attachment of surface unit have been detected in Biocoral group. The highest number of mineral like nodules have been observed in DDKD group on MC3T3-E1 cells. 71

Benzer Tezler

  1. Tez No

    630796

    Tideglusib ile karıştırılan kemik greftin yeni kemik oluşumu üzerine etkilerinin değerlendirilmesi

    The effect of tideglusib and bone graft combination on new bone formation

    BERRİN İYİLİKCİ

    Diş Hekimliği Uzmanlık

    Türkçe

    Türkçe

    2020

    Diş HekimliğiPamukkale Üniversitesi

    Ağız Diş ve Çene Cerrahisi Ana Bilim Dalı

    DR. ÖĞR. ÜYESİ OSMAN UFUK TAŞDEMİR

  2. Tez No

    90509

    Deri grefti ve diğer alloplastik greft materyalleriyle yapılan vestibuloplasti operasyonlarından sonra oluşan kontraksiyonun değerlendirilmesi

    Evaluation of wound contraction following vestibuloplasty operations, that skin graft and other alloplastic materials used

    YAVUZ EKİN

    Doktora

    Türkçe

    Türkçe

    2000

    Diş HekimliğiGazi Üniversitesi

    Ağız, Diş, Çene Hastalıkları ve Cerrahisi Ana Bilim Dalı

    PROF. DR. GÖKHAN H. ALPASLAN

  3. Tez No

    360124

    Farklı implant materyallerinin asellüler dermal matriks vaskülarizasyonuna etkileri

    Effects of different implant materials on vascularization of acellular dermal matrix

    SEVİN FARİZ

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2014

    Plastik ve Rekonstrüktif CerrahiKırıkkale Üniversitesi

    Cerrahi Tıp Bilimleri Bölümü

    DOÇ. DR. İLKER YAZICI

  4. Tez No

    420750

    Kemik defektlerinin kapatılmasında otojen kemik grefti, demineralize kemik matriksi (dbm), ß trikalsiyum fosfat (ß tcp) ve kombine dbm + trikalsiyum fosfat kullanımının karşılaştırılması

    Comparison of the usage of autogeneous bone graft, demineralized bone matrix (dbm), ß tricalcium phosphate (ß tcp) and combination of dbm + tricalcium phosphate for the closure of bone defects

    MELİKE ORUÇ

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2008

    Plastik ve Rekonstrüktif CerrahiSağlık Bakanlığı

    Plastik ve Rekonstrüktif Cerrahi Ana Bilim Dalı

    PROF. DR. UĞUR KOÇER

  5. Tez No

    732886

    Tavşan tibiasında deneysel olarak oluşturulan kemik defektlerinin değişik kemik greftleri ve tideglusib kullanılarak iyileşmenin radyolojik ve histopatolojik olarak değerlendirilmesi

    Radiological and histopathological evaluation of the healing of bone defects created experimentally in rabbit tibia by using various bone graft and tideglusib

    KEREM YENER

    Doktora

    Türkçe

    Türkçe

    2022

    Veteriner HekimliğiHarran Üniversitesi

    Cerrahi (Veterinerlik) Ana Bilim Dalı

    PROF. DR. ALİ HAYAT

Geri Dön