Alfa ve gamma Fe2O3 nanopartiküllere maruz bırakılan Daphnia magna'da nanotoksisite ve oksidatif stres etkilerinin belirlenmesi
Determination of the effects of oxidative stress and nanotoxicity on Daphnia magna exposed to alpha and gamma Fe2O3 nanoparticles
- Tez No: 526738
- Danışmanlar: DOÇ. DR. SELÇUK BERBER, DOÇ. DR. MEHMET ATEŞ
- Tez Türü: Yüksek Lisans
- Konular: Su Ürünleri, Aquatic Products
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2018
- Dil: Türkçe
- Üniversite: Çanakkale Onsekiz Mart Üniversitesi
- Enstitü: Fen Bilimleri Enstitüsü
- Ana Bilim Dalı: Su Ürünleri Temel Bilimleri Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 71
Özet
Nanopartikül (NP) (
Özet (Çeviri)
The products that include Nanoparticle (NP) are used in many fields, such as medical devices, electronics, the production and the storage of energy, health sciences and food sectors. Unfortunately, the knowledge in the literature is lack on the effects of NPs on the environment and human health. On the other hand, nanomaterials are commonly used in the wide range on half-conductive materials, metals and metal-oxides. This is because nanomaterials have a wide range, from metals, metal-oxides to half-conductive materials. In this study, nanotoxicity tests are conducted by means of exposing Daphnia magna, which is one of the primary zooplankton species living in freshwater ecosystem, to iron oxide (Fe2O3) NPs. The characterization of Fe2O3 NPs used in this study has been made with the methods of TEM, DLS, Zeta Potential, XRD, SEM, FT-IR and UV-Visible Spectroscopy. The measurement of NP-related accumulation and elimination rates has been made with ICP-MS analysis. The NP effects on zooplanktonic organisms has been monitored with phase-contrast microscopy. The toxic effects on zooplanktonic organisms have been examined with the GSH and TBARS, which are among mortality and oxidative stress symptoms. The results indicate that there are differences in the accumulation and the elimination rates of NPs, with differing influences of NPs on organisms. The accumulation in the organism increased with NP concentration. It has been observed that each NP made different metal ion oscillation in freshwater. The surfaces of the zooplankters which were exposed to NPs have been encapsulated with NPs. In oxidative stress analysis, TBARS value has increased in D. magna individuals, whereas ups and downs have been observed on the level of GSH. NPs taken by an organism constitue danger for the higher consumer.
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