诱导精神分裂症大鼠模型的诱发痛和自发痛评估
Başlık çevirisi mevcut değil.
- Tez No: 708732
- Danışmanlar: DR. JøRGEN SCHEEL-KRÜGER
- Tez Türü: Yüksek Lisans
- Konular: Nöroloji, Neurology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2022
- Dil: İngilizce
- Üniversite: Beijing Institute of Technology
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 71
Özet
些临床和研究表明，与正常人相比，精神分裂症患者对急性疼痛的敏感性降低，同时慢 性疼痛的发生率更高。但是，目前关于精神分裂症和疼痛的研究大多基于人类实验。除了 Boyette et. al. (2011) 采用机械痛评估精神分裂症模型大鼠的神经病理痛的疼痛行为外，还 没有基于对NMDAR拮抗剂介导的精神分裂症动物模型疼痛评估的研究。 本研究通过 MK-801 慢性给药在大鼠身上建立精神分裂症模型，并采用新颖客体识别任务， 旷场测试及糖水偏好测试用于检验精神分裂症模型的建立；同时检验了热痛及机械痛诱发 的 疼痛行为及福尔马林引起的自发性痛行为；结果显示，注射过 Mk -801 的大鼠对机械痛和热 痛的敏感性降低，对福尔马林引起的持续性疼痛的敏感性增加。此外，我们也采用了CFA 注 射诱导大鼠慢性炎症痛模型，并观察到经过 MK -801 给药的大鼠慢性疼痛持续时间更长。基 于这些发现，并考虑到疼痛与精神分裂症在皮质区域改变的相似性，我们初步探 讨了二者关 系的潜在机制。
Özet (Çeviri)
Several clinical and research reports indicated that Schizophrenia patients have decreased pain sensitivity to acute pain as well as high occurrence of chronic pain compared to normal individuals. Unfortunately, the information concerning the relationship between Schizophrenia and pain are mostly based on human studies. As far as known, there is only publication by Boyette et. al. (2011) that evaluated mechanical allodynia in NMDAR antagonism Schizophrenia rat model with neuropathic pain. The present study investigated pain sensitivities in distinct pain testing implementations in Sprague Dawley rats, which have been subchronically MK-801 administrated (0.5 mg/kg, twice a day for 7 days followed by a 7 days washout period) to present the translational appropriateness to mimic Schizophrenia-like behaviors. The validity of this model was presented with behavioral tests - novel object recognition test (NOR), sucrose preference test (SPT) and open field test (OFT) - in MK-801-injected rats prior to pain testing. The results revealed that MK-801- treated rats showed hyposensitivity to thermal-, and mechanical-induced evoked pain; hypoalgesia in phase I, and increased hyperalgesia in phase II of formalin-induced spontaneous pain; as well as increased thermal, and mechanical hyperalgesia in CFA-induced chronic pain model, compared to control rats. Moreover, the persistency of pain was observed with MK-801 treatment in the phase II of formalin test and thermal hyperalgesia in CFA-test. Based on these results, possible underlying mechanisms and cortical areas are mentioned by taking the similarities of pain and Schizophrenia into account.
