The effects of preservative methods on thecuticular hydrocarbons of forensically importantinsect species: Calliphora vomitoria
Başlık çevirisi mevcut değil.
- Tez No: 711929
- Danışmanlar: DR. HANNAH MOORE
- Tez Türü: Yüksek Lisans
- Konular: Adli Tıp, Forensic Medicine
- Anahtar Kelimeler: Forensic entomology, hydrocarbon analysis, preservation
- Yıl: 2017
- Dil: İngilizce
- Üniversite: Cranfield University
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 70
Özet
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Özet (Çeviri)
Estimation of post-mortem interval (PMI) is an important issue in crime investigations. In the field of forensic entomology, cuticular hydrocarbons analysis has been used for PMI determination, as in the case of cuticular hydrocarbons in Calliphora vomitoria to estimate the age of some species of fly larvae. Larvae generally abound in crime scenes and can provide the most useful information in predicting the post-mortem interval. With morphological techniques it is possible to identify 3rd instar blowfly larvae, although it may be more difficult to identify immature larvae. It is usually necessary to identify species by rearing larvae in adult flies. Cuticular hydrocarbons have the potential to predict the age of adult blowfly, especially when there is significance in forensic applications. Hydrocarbon analysis is considered to have an advantage over morphological identification techniques because it does not require that the larvae be 3rd instar. As a result, this method, which can provide species identification in immature larval stages, would be advantageous by greatly cutting the time required for PMI estimates at a critical stage of the criminal investigation. The aim of research project is to examine the effect of a preservative method (using 40%, 60%, 70% and 80% ethyl alcohol solutions and killing in near-boiling water prior to storage) on the cuticle hydrocarbon profiles of the forensically important blowfly C. vomitoria using gas chromatography–mass spectrometry (GC–MS), with the main focus being on the nonpolar hydrocarbon compounds. With the aid of this analysis, the chromatograms have been closely examined to see if there are any chemical changes of the hydrocarbon profile over time. In this way, the appropriate preservation conditions can be determined without deterioration of the hydrocarbon structure.
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