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Decontamination of sprouted seeds by high powerultrasound and irradiation

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  1. Tez No: 712078
  2. Yazar: OKTAY HAYKIR
  3. Danışmanlar: PROF. SACHA LUCCHİNİ, PROF. MOHáCSİNé FARKAS CSİLLA
  4. Tez Türü: Yüksek Lisans
  5. Konular: Endüstri ve Endüstri Mühendisliği, Industrial and Industrial Engineering
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2018
  8. Dil: İngilizce
  9. Üniversite: Szent István University
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 57

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Özet (Çeviri)

Finding the most efficient method to decontaminate fresh produce, including sprouts, from pathogenic microorganisms remains an unsolved problem as many knowledge gaps remain. The objective of this study was to assess the decontamination efficacy of ultrasound and irradiation treatments on mung bean and fenugreek sprouts. The efficacy of ultrasounds was evaluated on both mung bean seeds and sprouted seeds. The same procedure was then applied to fenugreek sprouts. Mung bean and fenugreek sprouts were exposed to ultrasound treatments (40 kHz, 240 W, 25 °C) for 10 min to investigate microbial reduction in total viable counts (TVC) and Enterobacteriaceae counts. We then evaluated the effects of irradiation treatments with doses of 1 kGy and 2 kGy on mung bean and fenugreek sprouts decontamination. Ultrasound treatment for 5 min caused 0.85 log CFU/g reduction in TVC on mung bean seeds. Ultrasound treatment for 5 min did not cause a significant reduction in Enterobacteriaceae count on mung bean sprouts (p > 0.05). Extending the treatment to 10 min resulted in 1.49 and 1.17 log CFU/g reductions of TVC and Enterobacteriaceae, respectively. However, there was no significant reduction in TVC and Enterobacteriaceae count on ultrasound treated fenugreek seeds for 10 min. In contrast, irradiation treatment caused significant reductions on mung bean and fenugreek sprouts in both (p < 0.05). There were 4.22 and 4.81 log CFU/g reductions in TVC, when mung bean sprouts were decontaminated by 1 kGy and 2 kGy of irradiation, respectively. For Enterobacteriaceae counts, the reductions were 4.29 and 5.65 log CFU/g for 1 kGy and 2 kGy, respectively. The microbial reductions on fenugreek sprouts were less than mung bean sprouts, but they were still significant. The treatment resulted in 2.93 and 3.78 log CFU/g reductions of TVC, when fenugreek sprouts were exposed to 1 kGy and 2 kGy of irradiation, respectively. Irradiation treatment with a dose of 2 kGy caused 3.13 and 4.31 log CFU/g reductions in Enterobacteriaceae count on fenugreek sprouts. The reductions with a dose of 2 kGy were higher than with a dose of 1 kGy. However, dose of 2 kGy was higher than the limits of U.S Food and Drug Administration (FDA) for fresh produce including sprouts (1 kGy). Irradiation treatment could be useful in reducing the population of pathogens on sprouted seeds. Ultrasound treatment was not effective as irradiation treatment. Ultrasound might be useful, but in combination with chemical sanitizers or other novel technologies to enhance microbial reduction of pathogens on sprouts.

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