Genome wide association analysis of free sugars in the storage roots of a diversecollection of carrot (Daucus carota L.)
Başlık çevirisi mevcut değil.
- Tez No: 718722
- Danışmanlar: DR. PHİLİPP W. SİMON
- Tez Türü: Yüksek Lisans
- Konular: Biyomühendislik, Ziraat, Bioengineering, Agriculture
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2020
- Dil: İngilizce
- Üniversite: University of Wisconsin-Madison
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 175
Özet
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Özet (Çeviri)
Carrot is one of the most widely consumed vegetables due to its culinary characteristics and health benefits. To ensure consumer acceptance, carrot breeders are primarily focused on improving appearance, but flavor is also important for consumers. Sugars are a major component of fresh carrot flavor and are the focus of this project. Carrot roots accumulate free sugars. Reducing sugar (glucose and fructose) and sucrose are the major free sugars (total sugars) in the carrot root. Rs is a single gene that controls the reducing sugar to sucrose balance in carrot roots with dominance for high percentage of reducing sugar. A naturally occurring recessive mutation prevents acid soluble invertase isozyme II transcript in the rs allele, causing high sucrose accumulation. Sugar content, and total dissolved solids (TDS) have been found to be correlated in previous studies. The objectives of this research were to evaluate the phenotypic variation for free sugars and total dissolved solids in a diverse collection of carrot accessions used in breeding programs and research, evaluate the relationship between domestication status, root color, and bolting status on free sugars and TDS of carrot accessions, and evaluate the global distribution of variation in percentage reducing sugar in this diverse collection. Carrot plant introductions (639 cultivated and 15 wild) from the USDA National Plant Germplasm System (NPGS) collection of Plant Introductions (PIs), 62 inbred lines from the United States Department of Agriculture (USDA), and University of Wisconsin-Madison carrot breeding programs, and 2 widely grown commercial hybrids were screened for free sugars and TDS, then a genome wide association study was performed to identify SNP markers. Northern and Southern Africa displayed average higher fructose, glucose, and percentage of RS whereas Western and Eastern Asia had an average higher sucrose. Accessions from Western Asia had the highest mean for total sugar while accessions from Southern Europe had the highest TDS. Cultivated PIs from Afghanistan, Turkey, United States, Russia, France, Germany, Hungary, India, Iran, Japan, China, and Netherlands had wide variation for reducing sugar whereas wild PIs had low reducing sugar values, suggesting that this mutation exists naturally. Orange-colored accessions had higher average fructose, glucose, and RS while whitecolored accessions had more sucrose. Purple, yellow, and white accessions had higher sugar and TDS. Non-bolting accessions had the higher sugar amount, and TDS. There was a moderate positive linear correlation with total sugar and TDS (r=0.48). The most significant candidate gene to be identified in this study was acid soluble invertase isozyme II (acid betafructofuranosidas, DCv3_Chr2.09563) which catalyzes the irreversible hydrolysis of sucrose into glucose and fructose. We also confirmed that this gene is a candidate for the Rs locus, as well as the presence of this gene on chromosome 2 on the carrot genome. A comparison of resequencing data of high and low RS samples by using IGV (Integrative Genomics Viewer) indicated that there are some SNPs and two insertions at the region of acid soluble invertase isozyme II gene on the genome. To our knowledge, this is the first large scale evaluation of free sugars and TDS in carrot, and first GWAS for free sugars and TDS in carrot.
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