Evaluation of anticancer potentials ofphysciosporin, a lichen-originated compound,in colorectal and breast cancer
Başlık çevirisi mevcut değil.
- Tez No: 719814
- Danışmanlar: PROF. JAE-SEOUN, HUR, PROF. HANGUN KİM
- Tez Türü: Doktora
- Konular: Göğüs Hastalıkları, Onkoloji, Chest Diseases, Oncology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2020
- Dil: İngilizce
- Üniversite: Sunchon National University
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 115
Özet
Özet yok.
Özet (Çeviri)
Lichens, which represent symbiotic associations of fungi and algae, are potential sources of numerous natural products. Physciosporin (PHY) is a potent secondary metabolite found in lichens and was recently reported to inhibit the motility of lung cancer cells via novel mechanisms. The present study investigated the anticancer potential of PHY on colorectal and breast cancer cells. PHY was isolated from lichen extract by preparative TLC. The effect of PHY on cell viability, motility and tumorigenicity was elucidated by MTT assay, hoechst staining, flow cytometric analysis, transwell invasion and migration assay, soft agar colony formation assay, Western blotting, qRT-PCR and PCR array in vitro as well as xi tumorigenicity study in vivo. In the first chapter, PHY decreased the viability of various colorectal cancer (CRC) cell lines (Caco2, CT26, DLD1, HCT116 and SW620). Moreover, PHY elicited cytotoxic effects by inducing apoptosis at toxic concentrations. At non-toxic concentrations, PHY dose-dependently suppressed the invasion, migration, and colony formation of CRC cells. PHY inhibited the motility of CRC cells by suppressing epithelial-mesenchymal transition and downregulating actin-based motility markers. In addition, PHY downregulated βcatenin and its downstream target genes cyclin-D1 and c-Myc. Moreover, PHY modulated KAI1 C-terminal-interacting tetraspanin and KAI1 expression and downregulated the downstream transcription factors c-jun and c-fos. Finally, PHY administration showed considerable bioavailability and effectively decreased the growth of CRC xenografts in mice without causing toxicity. Therefore, PHY suppresses the growth and motility of CRC cells via novel mechanisms. In the second chapter, effect of PHY was evaluated on breast cancer (BC) cells. we investigated the effects of PHY on growth, and metabolism of BC cells MCF-7 and MDA-MB-231. Our results indicated that PHY markedly inhibits BC cell growth. Cell cycle analysis and Annexin V-FITC/PI double staining showed that the toxic concentration of PHY induced the apoptosis on BC cells through the mitochondrial apoptotic pathway via the regulation of Bcl-2 family proteins BAX and Bcl-xL. In non-toxic concentration, PHY potently decreased the ability of migration, proliferation, and tumorigenesis in BC cells. Metabolic investigation xii revealed that PHY treatment significantly diminished the bioenergetic profile by reducing the glucose uptake, ATP generation, and lactate production. Moreover, PHY alters glucose metabolism-related gene expressions such as Glut1 and PKM2 and downregulated transcription factors and oncogenes related with PKM2 including β-catenin, c-Myc, HIF-1, NF-κB and STAT3. Orthotopic implantation mouse model of BC further confirmed that PHY treatment suppressed BC growth. In conclusion, present results indicated that PHY possessed significant anticancer activity against BC and CRC cell lines in vitro. Furthermore, PHY inhibited tumor growth in orthotopic and skin xenograft mouse models, respectively. Together, these findings suggest that PHY has a potential further in vivo application as an anti-proliferative, anti-glycolytic and anti-tumorigenic agent
Benzer Tezler
- Comparative evaluation of in vitro biological activities of chemically characterized tilia species: Protective effects against inflammation and cytotoxic effects on pancreatic cancer cells with 2d and 3d spheroid models
Kimyasal olarak karakterize tilia türlerinin in vitro biyolojik aktivitelerinin karşılaştırmalı değerlendirilmesi: Enflamasyona karşı koruyucu etkileri ve 2d ve 3d sferoid modeli ile pankreatik kanser hücrelerine sitotoksik etkileri
GAMZE YÜKSEL
Doktora
İngilizce
2022
Eczacılık ve FarmakolojiYeditepe ÜniversitesiFarmasötik Toksikoloji Ana Bilim Dalı
PROF. DR. HANDE SİPAHİ
DOÇ. DR. ETİL GÜZELMERİÇ
- Yeşil sentez yöntemiyle demir nanopartiküllerin bal fenolik ekstraktıyla sentezi ve biyolojik aktivitelerinin belirlenmesi
Synthesis of iron nanoparticles with honey phenolic extract and determination of their biological activities by the green synthesis method
CEYLAN AYYILDIZ
Yüksek Lisans
Türkçe
2023
BiyolojiBingöl ÜniversitesiMoleküler Biyoloji ve Genetik Ana Bilim Dalı
DOÇ. DR. BÜLENT KAYA
- Işınlayıcı dizileriyle doku-içi hipertermide enrji yoğunlaştırılması
Başlık çevirisi yok
ABDULLAH FERİKOĞLU
Doktora
Türkçe
1996
Elektrik ve Elektronik Mühendisliğiİstanbul Teknik ÜniversitesiPROF.DR. İNCİ AKKAYA
- Antikanser etkili yeni hidrazon türevlerinin sentezi, biyolojik aktiviteleri ve yapı-etki ilişkilerinin değerlendirilmesi
Synthesis of new hydrazone derivatives with anti-cancer effect, evaluation of their biological activities and structure-actity relationships
ZAHRA MARYAM
Yüksek Lisans
Türkçe
2024
Eczacılık ve FarmakolojiAnadolu ÜniversitesiFarmasötik Kimya Ana Bilim Dalı
PROF. DR. ZAFER ASIM KAPLANCIKLI
- Yeni benzazol türevi bileşiklerin tasarımı, sentezi, yapılarının aydınlatılması, antiproliferatif aktivitelerinin araştırılması ve yapı-etki ilişkilerinin belirlenmesi
Design, synthesis, evaluation of structures, investigation of antiproliferative activities, and determination of structure-activity relationships of novel benzazole derivatives
BAYAN ZOATIER