Relation of the micro-RNA content of unsorted cryopreserved sperm and the fertility of sperm after sex-sorting
Başlık çevirisi mevcut değil.
- Tez No: 721178
- Danışmanlar: PROF. DR. HEİNRİCH BOLLWEİN, PROF. DR. HARALD SİEME
- Tez Türü: Doktora
- Konular: Veteriner Hekimliği, Zooloji, Veterinary Medicine, Zoology
- Anahtar Kelimeler: bull fertility, sex-sorted sperm, microRNA, small RNA-seq
- Yıl: 2020
- Dil: İngilizce
- Üniversite: Universität Zürich
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 55
Özet
Es wurde überprüft, ob die Fertilität von kryokonserviertem, gesextem Bullensperma anhand der micro-RNA (miRNA) in konventionell hergestelltem Tiefgefriersperma beurteilt werden kann. Dazu wurde Sperma von 18 Bullen ausgewählt, bei denen die Fertilität anhand der Non-Return Rate bekannt war (NRRconv u. NRRss für konventionelles bzw. für gesextes Sperma). Die Bullen wiesen ähnliche NRRconv (69.09%±2.82%) auf. Bei 9 Bullen war die NRRss deutlich gegenüber der NRRconv verringert (D = 22.33%±4.01%; Low Fertility = LF), während bei den anderen 9 Bullen die NRRss im Vergleich zur NRRconv nur moderat reduziert war (D = 8.63%±1.71%; High Fertility = HF)). Für die Sequenzierung der miRNAs wurden von jedem Bullen Proben je 4 Ejakulate gepoolt. Es wurden 85 miRNAs identifiziert, wobei die miR-34b-3p und miR-100-5p am stärksten und die miR-10a-5p und miR-9-5p zwischen den Ejalukaten von HF- und LF-Bullen unterschiedlich stark exprimiert waren (P0.05). Die miR-34c, miR-7859, miR-342, miR-106b-5p und miR-92a waren am aussagekräftigsten zur Prognose der NRRss (robuste Regressionsanalyse). Zusammenfassend lieferten die miRNAs konventionell hergestellten Tiefgefrierspermas wertvolle Hinweise auf die Fruchtbarkeit gesexten Bullenspermas. Schlüsselwörter: Bullenfruchtbarkeit; gesextes Sperma; microRNA; small RNA-Seq
Özet (Çeviri)
In this study, we hypothesised that the microRNA (miRNA) profile of cryopreserved unsorted (conventional) bovine sperm is related to bull fertility after artificial insemination (AI) with X-bearing sperm. Eighteen bulls were selected based on their annual 56-day non-return rate after AI with conventional (NRRconv) and sex-sorted sperm (NRRss), and assigned to two equal groups: low- (LF) and high-fertility (HF). The LF and HF bulls had similar NRRconv (69.09%±2.82%) but their NRRss was reduced by 22.33%±4.01% and 8.63%±1.71%, respectively. Small RNA libraries prepared from a pool of four ejaculates per bull were sequenced with an Illumina HiSeq 2500 system. The Spearman's rank correlation coefficient (rs) was used to evaluate the relation between NRR values and miRNA expression levels; the value of the latter as predictor of NRRss was assessed with robust regression analysis. Out of 85 totally detected miRNAs, miR-34b-3p and miR-100-5p were the two most abundant. MiR-10a-5p and miR-9-5p were differentially expressed in LF and HF samples (false discovery rate0.05). MiR-34c, miR-7859, miR-342, miR-106b-5p and miR-92a showed the highest contribution to the prediction of NRRss. Concluding, bull fertility after AI with sex-sorted sperm appeared to be linked with the miRNA profile of sperm before sorting.
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