Distribution and vertical transmission of southern tomato virusin Tomato
Güney domates virüsünün Tomato'da dağılımı ve dikey bulaşması
- Tez No: 764790
- Danışmanlar: PROF. DR. PLANT PATHOLOGY
- Tez Türü: Yüksek Lisans
- Konular: Ziraat, Agriculture
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2017
- Dil: İngilizce
- Üniversite: Unıversıty Of Florıda
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: Belirtilmemiş.
Özet
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Özet (Çeviri)
Southern tomato virus (STV) is a recently identified virus that is present in tomato cultivars worldwide. STV is a monopartite virus with a double-stranded RNA genome (3.5 kb) containing two overlapping open reading frames (ORFs) and is the type species of the genus Amalgavirus (family Amalgaviridae). A reverse-transcription polymerase chain reaction (RT-PCR) using virus-specific primers was used to detect STV in 24 tomato cultivars, which included hybrid and open pollinated cultivars. STV was detected in plants produced by a number of seed companies, indicating that the virus is widely distributed in the vegetable seed industry. STV was detected for the first time in the following cultivars: 'Agriset 761', 'Bonita', 'Florida Lanai', 'Mexico Midget', and 'Roma'. Partial isolates of STV from this study and full genome sequences of STV from GenBank were compared and found to have an average percentage diversity of less than 1%. In order to look at the presence of STV in different developmental stages of tomato plants, STV-infected and STV-negative 'Sweet Hearts' tomato plants were grown and sampled for the presence of STV using RT-PCR at different developmental stages. STV was detected in 75% of 1 to 2 week-old germinated seedlings. When plants were grown and sampled at various leaf stages up to final sampling at 15th leaf stage, all positive plants were positive for STV and the negative plants remained negative. The presence or absence of STV was consistent across all four developmental stages. Seed transmission studies of progeny produced from crosses of different combinations of parents that were STV-infected or -negative, revealed that both ovule and pollen transmission of STV occurred at high rates
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