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Establishing a yeast-based system for investigating human apoptotic regulators

Başlık çevirisi mevcut değil.

  1. Tez No: 853257
  2. Yazar: EMİNE KAZANÇ
  3. Danışmanlar: Belirtilmemiş.
  4. Tez Türü: Yüksek Lisans
  5. Konular: Biyomühendislik, Mikrobiyoloji, Moleküler Tıp, Bioengineering, Microbiology, Molecular Medicine
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2016
  8. Dil: İngilizce
  9. Üniversite: University of Leicester
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 60

Özet

Apoptosis is a significant property of normal somatic cells. However, cancer cells lose this important function as one step to becoming immortalized. Human apoptosis is also regulated by a number of protein families, for example, the Bcl-2 family is composed of a number of pro-apoptotic members (e.g. Bax) and anti-apoptotic members (e.g. Bcl-2 and Bcl-xL). Although Baker's yeast (Saccharomyces cerevisiae) cells do not expresses Bcl-2 family proteins, they undergo apoptosis in response to the heterogeneous expression of human Bax. The strength of Bax isoforms to induce apoptosis in yeast may be different and such knowledge combined with chromosome-editing techniques could generate novel yeast strains, which can be used to detect potential apoptotic regulators in an efficient way. In this study we aimed in our study to establish a novel yeast system based on three human selected Bax isoforms including Baxα, Baxβ and Baxσ, to screen potential apoptotic regulators or to investigate potential roles of interesting proteins in apoptosis. As the result, three new yeast expression plasmids (under the control of a galactose promoter), containing N-terminally HA-tagged and C-terminally Myc-tagged Baxα, Baxβ or Baxσ were generated successfully. In addition, using yeast chromosome editing techniques, the defective GAL3 gene in Y55 has been repaired with that in W303. Both Y55 and W303 are well-used yeast strains in laboratory. The data presented here indicated that a novel yeast system was potentially established, which could be used in cancer studies to investigate or screen potential apoptotic regulators efficiently.

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