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üropatojen escherichia coli suşlarında ve barsak florasında bulunan kommensal escherichia coli suşlarında PAP, SFA ve AFA adezinleri kodlayan operonların saptanması

Detection of PAP, SFA and AFA adhesin-encoding operons in uropathogenic escherichia coli strains and commensal escherichia coli strains of the gut flora

  1. Tez No: 131812
  2. Yazar: NEŞE KİREMİT KORKUT
  3. Danışmanlar: PROF. DR. MİNE ANĞ KÜÇÜKER
  4. Tez Türü: Doktora
  5. Konular: Mikrobiyoloji, Microbiology
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2003
  8. Dil: Türkçe
  9. Üniversite: İstanbul Üniversitesi
  10. Enstitü: Sağlık Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Mikrobiyoloji ve Klinik Mikrobiyoloji Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 80

Özet

7. SUMMARY E. coli is the major causative agent of the urinary tract infections, which are the most encountered infectious diseases in humans. Adhesion is the first step of the urinary tract infections and it is the function of fimbria! adhesins like pap and sfa and nonfimbrial adhesins like afa. Therefore the determination of the existence of these adhesins has diagnostic and therapeutic importance. The aim of this study is to determine the operons of the pap, sfa and afa adhesins in 50 causative E. coli strains isolated from urine of the patients with a urinary tract infection and 50 strains isolated from the faeces of healthy humans. The operons of the pap, sfa and afa adhesins are studied using multiplex PCR; a specific and practical method. Of the isolates from the patients with urinary tract infection; 8 (%16) were pap positive, 4 (%8) were afa positive, 4 (%8) were afa and pap positive, and 1 (%2) was afa + pap positive. Of the isolates from the faeces of healthy humans, 4 (%8) were pap positive, 2 (%4) were sfa positive and 1 (%2) was afa positive. Compared eachother, the difference of the percentages of the operons was found statistically significant. 58

Özet (Çeviri)

9. KAYNAKLAR 1) Abraham JM, Freitag CS, Clements JR, Eisenstein Bl: An invertible element of DNA controls phase variation of type I fimbriae of Escherichia coli. Proc Natl Acad Sci USA 1985; 82: 5724 - 5727. 2) Aim RA, Mattick JS. Genes involved in the biogenesis and function of type-4 fimbriae in Pseudomonas aeruginosa. Gene 1997; 192: 89 - 98. 3) Archambaud M, Courcoux P, Quin V, Chabanon G, Labigne-Roussel A. Phenotypic and genotypic assays for the detection and identification of adhesins from pyelonephritic Escherichia coli. Ann Inst Pasteur Microbiol 1988; 139:557-573. 4) Babai R, Stern BE, Hacker J, Ron EZ. New fimbrial gene cluster of S fimbrial adhesin family. Infect Immun 2000; 68: 5901 - 5907. 5) Balague CE, Ruiz CS, Rey R, Duffard AME, Neder-Macias ME. Effect of the herbicide 2,4-dichlorophenoxyacetic acid on uropathogenic Escherichia coli virulence factors. Toxicology 2002; 177: 143 - 155. 6) Baron EJ, Peterson LR, Finegold SM. Bailey and Scott's Diagnostic Microbiology, 9th edition. Mosby Publisher, Toronto, 1994; 362 - 365. 7) Ben Nasr A, Olsen A, Sjöbring U, Miiller-Esteri W, Björk L Assembly of human contact phase proteins and release of bradykinin at the surface of curli- expressing Escherichia coli. Mol. Microbiol 1996. 20: 927-935. 8) Bian Z, Normark S. Nucieator function of CsgB for the assembly of adhesive surface organelles in Escherichia coli. EMBO J 1997; 16: 5827 - 5836. 59

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