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X-Kromozomal STR Polimorfizmi (DXS8377, DXS101X DXS6789, STR-1, HUMHPRTB) ve Türk Toplumundaki Alel Freskansları

X-Chromosomal STR pOLYMORPHİSM (DXS8377, DXS101X DXS6789, STR-1, HUMHPRTB) and Turkish Allele Frequencies

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  1. Tez No: 192908
  2. Yazar: FARUK AŞICIOĞLU
  3. Danışmanlar: PROF.DR. MAHMUT N. ÇARİN
  4. Tez Türü: Doktora
  5. Konular: Tıbbi Biyoloji, Medical Biology
  6. Anahtar Kelimeler: 675 JRQRVRPDO 675 SDWHUQLW\ WHVWL DNUDEDOÕN DQDOL]L DGOLgenetik, X-STRs, gonosomal STRs, paternity testing, kinship analysis, forensicgenetics
  7. Yıl: 2006
  8. Dil: Türkçe
  9. Üniversite: İstanbul Üniversitesi
  10. Enstitü: Sağlık Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Tıbbi Biyoloji Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: Belirtilmemiş.

Özet

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Özet (Çeviri)

ABSTRACT$úÕFÕR÷OX ) X-Chromosomal STR Polymorphism (DXS8377, DXS101,DXS6789, STRX-1, HUMHPRTB) and Turkish Allele Frequencies, Institute of Health6FLHQFH0HGLFDO%LRORJ\3K'7KHVLVøVWDQEXOA large number of autosomal DNA polymorphisms have been evaluated forforensic use and widely applied to stain analysis and kinship testing. In addition,forensic interest has increasingly focused on Y-chromosomal markers over the past fewyears. So far, X linked STRs have received little attention in forensic literature. The aimof this study is to determine the allele frequencies of five X-chromosomal STR loci, andto evaluate their efficiency in forensic practice. DNA was extracted from the blood ofunrelated Turkish citizens (126 females, 139 males) using 5% Chelex 100. PCRamplification was performed using fluorecently labeled primers for DXS8377, DXS101,DXS6789, STRX-1, HUMHPRTB loci, sequenced based on gene bank information.The PCR touch down protocol was chosen. The PCR products were analysed bycapillary electrophoresis by ABI Prism 310. DXS8377 and DXS101 loci have both 18alleles following by DXS1(11 alleles), DXS6789(10)alleles, and HPRTB(8 alleles),respectively. DXS8377 and DXS101 loci have both very high, PIC, MEC and PDvalues, respectively. In contrast, DXS6789 has the lowest values from this aspect.PD(female) values are higher than PD(male) values at all loci. The allele frequencies inthis study showed very similar distributions with some population such as German,$XVWULDQ 6SDQLVK øWDOLDQ DQG 3ROLVK ;FKURPRVRPH 675V DUH KLJKO\ LQIRUPDWLYHmarkers for kinship testing and especially important in deficiency cases, because themean exclusion chance of X-STRs tends to be higher than that of autosomal STR loci.

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