Geri Dön

Mezenter iskemi reperfüzyon hasarı üzerine silimarinin etkisi

The effect of silymarin on intestinal ischemia-reperfusion injury

PDF İndir

  1. Tez No: 297345
  2. Yazar: MÜMİN DEMİR
  3. Danışmanlar: PROF. DR. CAFER POLAT
  4. Tez Türü: Tıpta Uzmanlık
  5. Konular: Genel Cerrahi, General Surgery
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2011
  8. Dil: Türkçe
  9. Üniversite: Ondokuz Mayıs Üniversitesi
  10. Enstitü: Tıp Fakültesi
  11. Ana Bilim Dalı: Genel Cerrahi Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 73

Özet

AMAÇ: Akut mesenter iskemi acil girişim gerektiren karın hastalıklarının %1-2 sini oluşturmaktadır. Silimarin'in böbrek, karaciğer ve kalp dokusunda iskemi -reperfüzyon hasarını azalttığı rapor edilmiştir. İntestinal sistemde, akut mesenter iskemi hastalığında ve ince barsak transplantasyonu sürecinde iskemi reperfüzyon hasarı yaşanmaktadır. Bu çalışmanın amacı ratlarda silimarinin mezenter iskemi-reperfüzyon hasarı üzerine etkilerini araştırmaktır.MATARYEL VE METOT: Çalışmamızda 5 grupta 10 ar adet yetişkin Wistar Albino rat kullanıldı. Birinci grupta(sham grubu) deneklere sadece laparatomi yapıldı. İkinci grupdaki deneklere (kontrol grubu) süperior mezenterik arter klemplendi. 60 dk iskemi yapıldı ve sonrasında 24 saatlik reperfüzyon sağlandı. Üçüncü grupta 1 saat iskemi, sonrası reperfüzyon sağlandı. Yedi gün, günde bir kez 100 mg /kg oral silimarin verildi. Dördüncü grupta, 7 gün süreyle aynı dozda silimarin verildikden sonra 1 saat mezenter iskemi yapıldı ve 24 saat reperfüzyon sağlandı. Beşinci grupta iskemiden 7 gün önce ve 7 gün sonra aynı dozda silimarin verildi. Tüm deneklerden kan alındı ve iskemi-reperfüzyon hasarı göstergesi olarak oksidatif stres belirteçleri (Hsp 70, süperoksit dismutaz (SOD), malondialdehit (MDA), protein karbonil) ve proinflamatuvar sitokin TNF-? düzeyleri çalışıldı. Terminal ileumdan patolojik inceleme için doku örnekleri alındı.BULGULAR: Serum Hsp 70 düzeyleri kontrol grubuna kıyasla silimarin verilen gruplarda yüksek bulundu (p< 0.05). Kontrol grubuna kıyasla SOD düzeyleri silimarin verilen gruplarda düşük bulundu (p

Özet (Çeviri)

Background: Although the mechanism is unknown, Silymarin is shown to have protective effect against kidney, liver, and heart ischemia and reperfusion injury. In this study, the effect of silymarin on intestinal ischemia-reperfusion injury is evaluated.Material and Method: 50 Wistar Albino rats, were divided in to five egual groups. Group I: Sham group, only laparotomy was performed. Group II: Control group, superior mesenteric artery was clamped for 60 minutes and then reperfused for 24 hours. Group III: Superior mesenteric artery was clamped for 60 minutes and then reperfused. Rats were fed with oral 100 mg /kg silymarin for 7 days and were sacrified at 8th day. Group IV: Rats were fed with oral 100 mg /kg silymarin for 7 days and then superior mesenteric artery was clamped for 60 minutes. Rats were sacrified after 24 hour reperfusion. Group V: Rats were fed with oral 100 mg /kg silymarin for 7 days and then superior mesenteric artery was clamped for 60 minutes. Rats were fed with oral 100 mg /kg silymarin for 7 days after ischemia and sacrified at 8th day.Blood were withdrawn during sacrification to study the bood levels of anti-oxidant parameters such as Hsp 70, superoxidet dismutase (SOD), malondealdehyde (MDA), protein carbonyl and proinflammatory cytokines (TNF-?). Terminal ileum was removed for pathological evaluation.Results: Blood Hsp 70 levels were higher in the silymarin given groups than the control group (p< 0.05). SOD levels were lower in the silymarin given groups than the control group (p< 0.05). SOD levels were lowest in Group V (p< 0.002). There was no statisctical difference in malondealdehyde (MDA) and protein oxidation (protein carbonyl) levels among the groups. There was statistically significant difference in TNF-? levels between control group and Grup IV (p< 0.05).Pathological evaluation showed control group had significant morphological changes than the other groups else sham group. Morphological changes were lower in rats received silymarin. This change was prominent in Group V (p< 0.01).Result: Silymarin have anti-inflammatory and anti-oxidant effect on mesenteric ischemia reperfusion damage. This effect is prominent when given before and after ischemia.ABSTRACTBackground: Although the mechanism is unknown, Silymarin is shown to have protective effect against kidney, liver, and heart ischemia and reperfusion injury. In this study, the effect of silymarin on intestinal ischemia-reperfusion injury is evaluated.Material and Method: 50 Wistar Albino rats, were divided in to five egual groups. Group I: Sham group, only laparotomy was performed. Group II: Control group, superior mesenteric artery was clamped for 60 minutes and then reperfused for 24 hours. Group III: Superior mesenteric artery was clamped for 60 minutes and then reperfused. Rats were fed with oral 100 mg /kg silymarin for 7 days and were sacrified at 8th day. Group IV: Rats were fed with oral 100 mg /kg silymarin for 7 days and then superior mesenteric artery was clamped for 60 minutes. Rats were sacrified after 24 hour reperfusion. Group V: Rats were fed with oral 100 mg /kg silymarin for 7 days and then superior mesenteric artery was clamped for 60 minutes. Rats were fed with oral 100 mg /kg silymarin for 7 days after ischemia and sacrified at 8th day.Blood were withdrawn during sacrification to study the bood levels of anti-oxidant parameters such as Hsp 70, superoxidet dismutase (SOD), malondealdehyde (MDA), protein carbonyl and proinflammatory cytokines (TNF-?). Terminal ileum was removed for pathological evaluation.Results: Blood Hsp 70 levels were higher in the silymarin given groups than the control group (p< 0.05). SOD levels were lower in the silymarin given groups than the control group (p< 0.05). SOD levels were lowest in Group V (p< 0.002). There was no statisctical difference in malondealdehyde (MDA) and protein oxidation (protein carbonyl) levels among the groups. There was statistically significant difference in TNF-? levels between control group and Grup IV (p< 0.05).Pathological evaluation showed control group had significant morphological changes than the other groups else sham group. Morphological changes were lower in rats received silymarin. This change was prominent in Group V (p< 0.01).Result: Silymarin have anti-inflammatory and anti-oxidant effect on mesenteric ischemia reperfusion damage. This effect is prominent when given before and after ischemia.ABSTRACTBackground: Although the mechanism is unknown, Silymarin is shown to have protective effect against kidney, liver, and heart ischemia and reperfusion injury. In this study, the effect of silymarin on intestinal ischemia-reperfusion injury is evaluated.Material and Method: 50 Wistar Albino rats, were divided in to five egual groups. Group I: Sham group, only laparotomy was performed. Group II: Control group, superior mesenteric artery was clamped for 60 minutes and then reperfused for 24 hours. Group III: Superior mesenteric artery was clamped for 60 minutes and then reperfused. Rats were fed with oral 100 mg /kg silymarin for 7 days and were sacrified at 8th day. Group IV: Rats were fed with oral 100 mg /kg silymarin for 7 days and then superior mesenteric artery was clamped for 60 minutes. Rats were sacrified after 24 hour reperfusion. Group V: Rats were fed with oral 100 mg /kg silymarin for 7 days and then superior mesenteric artery was clamped for 60 minutes. Rats were fed with oral 100 mg /kg silymarin for 7 days after ischemia and sacrified at 8th day.Blood were withdrawn during sacrification to study the bood levels of anti-oxidant parameters such as Hsp 70, superoxidet dismutase (SOD), malondealdehyde (MDA), protein carbonyl and proinflammatory cytokines (TNF-?). Terminal ileum was removed for pathological evaluation.Results: Blood Hsp 70 levels were higher in the silymarin given groups than the control group (p< 0.05). SOD levels were lower in the silymarin given groups than the control group (p< 0.05). SOD levels were lowest in Group V (p< 0.002). There was no statisctical difference in malondealdehyde (MDA) and protein oxidation (protein carbonyl) levels among the groups. There was statistically significant difference in TNF-? levels between control group and Grup IV (p< 0.05).Pathological evaluation showed control group had significant morphological changes than the other groups else sham group. Morphological changes were lower in rats received silymarin. This change was prominent in Group V (p< 0.01).Result: Silymarin have anti-inflammatory and anti-oxidant effect on mesenteric ischemia reperfusion damage. This effect is prominent when given before and after ischemia.ABSTRACTBackground: Although the mechanism is unknown, Silymarin is shown to have protective effect against kidney, liver, and heart ischemia and reperfusion injury. In this study, the effect of silymarin on intestinal ischemia-reperfusion injury is evaluated.Material and Method: 50 Wistar Albino rats, were divided in to five egual groups. Group I: Sham group, only laparotomy was performed. Group II: Control group, superior mesenteric artery was clamped for 60 minutes and then reperfused for 24 hours. Group III: Superior mesenteric artery was clamped for 60 minutes and then reperfused. Rats were fed with oral 100 mg /kg silymarin for 7 days and were sacrified at 8th day. Group IV: Rats were fed with oral 100 mg /kg silymarin for 7 days and then superior mesenteric artery was clamped for 60 minutes. Rats were sacrified after 24 hour reperfusion. Group V: Rats were fed with oral 100 mg /kg silymarin for 7 days and then superior mesenteric artery was clamped for 60 minutes. Rats were fed with oral 100 mg /kg silymarin for 7 days after ischemia and sacrified at 8th day.Blood were withdrawn during sacrification to study the bood levels of anti-oxidant parameters such as Hsp 70, superoxidet dismutase (SOD), malondealdehyde (MDA), protein carbonyl and proinflammatory cytokines (TNF-?). Terminal ileum was removed for pathological evaluation.Results: Blood Hsp 70 levels were higher in the silymarin given groups than the control group (p< 0.05). SOD levels were lower in the silymarin given groups than the control group (p< 0.05). SOD levels were lowest in Group V (p< 0.002). There was no statisctical difference in malondealdehyde (MDA) and protein oxidation (protein carbonyl) levels among the groups. There was statistically significant difference in TNF-? levels between control group and Grup IV (p< 0.05).Pathological evaluation showed control group had significant morphological changes than the other groups else sham group. Morphological changes were lower in rats received silymarin. This change was prominent in Group V (p< 0.01).Result: Silymarin have anti-inflammatory and anti-oxidant effect on mesenteric ischemia reperfusion damage. This effect is prominent when given before and after ischemia.

Benzer Tezler

  1. Tez No

    99065

    İntestinal iskemi reperfüzyon hasarı üzerine N-Asetilsistein'in etkisi

    The Effect of N-Acetylcystein on the intestinal ischemia-reperfusion injury

    K. FERDA DÜLGER

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2000

    Genel CerrahiAnkara Üniversitesi

    Genel Cerrahi Ana Bilim Dalı

    DOÇ.DR. NEZİH ERVERDİ

  2. Tez No

    479924

    Sıçanlarda Ganoderma lucidumun mezenter iskemi- reperfüzyon hasarı üzerindeki iyileştirici etkileri

    The healing effects of ganoderma lucidum on mesenteric ischemia-reperfusion injury in rats

    MUHAMMED GÖMEÇ

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2017

    Genel CerrahiBozok Üniversitesi

    Genel Cerrahi Ana Bilim Dalı

    PROF. DR. BAHADIR KÜLAH

  3. Tez No

    248360

    Sıçanlarda abdominal kompartman sendromu modelinde dopamin infüzyonunun mezenterik kan akımı ve iskemi-reperfüzyon hasarı üzerine etkisi

    Başlık çevirisi yok

    AYTEN SARAÇOĞLU ÇAKAR

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2008

    Anestezi ve ReanimasyonMarmara Üniversitesi

    Anesteziyoloji ve Reanimasyon Ana Bilim Dalı

  4. Tez No

    830037

    Barsak iskemi-reperfüzyon hasarı üzerine leptinin etkisi

    Effects of leptin on intestinal ischemia-reperfusion injury

    SEVGİ BÜYÜKBEŞE SARSU

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2006

    Çocuk CerrahisiGaziantep Üniversitesi

    Çocuk Cerrahisi Ana Bilim Dalı

    PROF. DR. BÜLENT HAYRİ ÖZOKUTAN

  5. Tez No

    501961

    Ratlarda deneysel mezenter iskemi reperfüzyon hasarı modelinde düşük molekül ağırlıklı heparinlerin bakteriyel translokasyon üzerine etkileri

    The effects of low molecular weight heparins on bacterial translocation in experimental mesenter ischemia reperfusion injury model

    SELÇUK KÖKSAL

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2018

    Genel CerrahiBolu Abant İzzet Baysal Üniversitesi

    Genel Cerrahi Ana Bilim Dalı

    PROF. DR. NURİ AYDIN KAMA

Geri Dön