Computer interfaced CCD camera
Başlık çevirisi mevcut değil.
- Tez No: 364442
- Danışmanlar: PROF. DR. YUSUF TAN
- Tez Türü: Yüksek Lisans
- Konular: Biyomühendislik, Bioengineering
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 1992
- Dil: İngilizce
- Üniversite: Boğaziçi Üniversitesi
- Enstitü: Biyo-Medikal Mühendislik Enstitüsü
- Ana Bilim Dalı: Biyomedikal Mühendisliği Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 144
Özet
Özet yok.
Özet (Çeviri)
This thesis work is aimed at the building of a computer interfaced CCD (Charge Coupled Device) camera; which will be part of a fluorescence ratio imaging system, measuring cytosolic free Ca++ concentration. As in the current systems, for ratio imaging of rapidly responding cells, the incompatibility with the standard video is a serious problem the analog video output is directly digitised using a flash ADC (Analog to Digital Converter); and 7-bit digital data is transferred to the computer memory using DMA (Direct Memory Access). So, the data acquisition rate is only limited by the CCD and the computer performance. The system is designed around an IBM compatible PC/AT. Electronic hardware is composed of four card connected to PC via the expansion cards: an adapter slots, a voltage regulator , an ADC and a CCD card. The mechanical hardware is simply a light isolated housing carrying the last three cards. It is coupled to a student microscope with removed oculaire to give a real image on the CCD surface. The charge packages are collected at the potential wells of CCD which are clocked out by supplying the gating pulses. The gating pulses together with the DMA control signals are generated at the adapter card.The software initiates the data acquisition process. When a word (two bytes) of data is ready, the PAL (Programmable Logic Array) produces a DRQ (DMA Request) signal. When the data is transferred to memory PC sends a DACK (DMA Acknowledge) signal. PAL holds the clocking out of the CCD until DACK comes. Once all of the pixels are clocked out, PC sends a TC (Termination Count) signal which in turn generates a hardware interrupt. Once this interrupt is recognised by PC: a new data acquisition sequence will start; but this time on a second buffer leaving the first buffer available for any real time data processing; thereby creating a kind of background task. The limiting frame rate of the camera is 100 Hz; while with the 18 MHz PC resulting images are obtained at 33 Hz. The processed images are 64 gray level black-and-white or pseudocolored images. Black-and-white images were used to focus on the target cells. pseudocolored ones will be used to relate the light intensity to a local analyte concentration.
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