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Identification of expression quantitative trait loci (eQTL) and candidate genes associated with water holding capacity in porcine meat

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  1. Tez No: 400398
  2. Yazar: MEHMET ULAŞ ÇINAR
  3. Danışmanlar: PROF. DR. KARL SCHELLANDER
  4. Tez Türü: Doktora
  5. Konular: Zooloji, Zoology
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2010
  8. Dil: İngilizce
  9. Üniversite: Rheinische Friedrich-Wilhelms-Universität Bonn
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 129

Özet

Die Beurteilung von Fleischqualität durch den Konsumenten ist definiert durchsensorische Merkmale, wie Saftigkeit, Muskel pH, Wasserhaltevermögen und Farbe.Das Wasserhaltevermögen in Bezug auf den Tropfsaftverlust hat sowohl eine genetischeals auch eine ökologische Komponente mit geringer bis mittlerer Heritabilität. DieAnzahl der Gene die an dem Merkmal Tropfsaftverlust beteiligt sind, sind bislang nochunbekannt. Das Ziel dieser Studie war es, geeignete Kandidatengene, die für denTropfsaftverlust im Schweinefleisch verantwortlich sind, zu identifizieren.Für die Kandidatengenanalyse und ihre eQTL Positionen wurden zwanzig Geneselektiert. Zur Erstellung der Expressionsprofile von 300 DUPI Tieren wurde einerseitseine quantitative Real-Time PCR verwendet, sowie das GenomeLab GeXP MultiplexVerfahren. Zur Normalisierung der Expression wurden mehrere Referenz Geneeingesetzt. Die Analyse zeigte das die Gene Peroxisome proliferator activated receptorgamma, coactivator 1 alpha (PPARGC1) und Alpha 1 microglobulin/bikunin (AMBP) inVergleich zwischen hohem und niedrigem Tropfsaftverlust unterschiedlichexprimierten. Darüber, hinaus zeigte die Expression weiterer Gene signifikanteAssoziationen mit verschiedenen Parametern der Fleischqualität. Die Analyse der eQTLerbrachte das diese Gene in der DUPI Population trans-reguliert waren. Durch dieVerwendung verschiedener QTL Modelle wurde auf SSC2 in der Nähe des MarkersS0141 eine vielversprechende chromosomale Region für Tropfsaftverlust entdeckt, dieskonnte durch Fachliteratur bestätigt werden. Durch die Übereinstimmung entdecktereQTL mit QTL in der DUPI Population und in anderen Schweine Populationen konntenweitere vielversprechende chromosomale Regionen für zukünftige Feinkartierungenund Assoziationsstudien a werden.

Özet (Çeviri)

Consumer assessment of meat quality is defined by the characteristics of sensoryexperience such as juiciness, muscle pH, water-holding capacity and colour. Waterholdingcapacity in terms of drip loss has a genetic as well as environmental componentwith low to medium heritability. The number of genes involved in the development ofdrip loss is unknown. The aim of this study was to identify the candidate genes and theirtranscriptional regulation responsible for the drip loss in pig meat.Twenty genes were selected for the candidate gene analysis and for their eQTL study.For the expression of genes quantitative real-time PCR and GenomeLab GeXP multiplexwere used in 300 DUPI animals. Multiple housekeeping genes were used for theaccurate gene expression normalization. Analysis revealed expression of peroxisomeproliferator activated receptor gamma, coactivator 1 alpha (PPARGC1) and alpha 1microglobulin/bikunin (AMBP) genes were differentially regulated in animals withhigher drip loss compared to lower drip loss. Moreover, expression of other genesshowed significant association with different meat quality parameters. eQTL analysisshowed that these genes are trans-regulated in DUPI population. By using differentQTL models, on SSC2 vicinity of marker S0141 was detected as the most promisingchromosomal region for drip loss supported by the literature as well. Ovelapping ofdetected eQTL with QTL in DUPI population and other pig populations showedpromising chromosomal regions for further fine mapping and association studies.

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