The induced resistance against fire blight by the plant activator BTH (BION®) or extract of Hedera helix leaves and studies on the mode of action
Başlık çevirisi mevcut değil.
- Tez No: 400406
- Danışmanlar: PROF. DR. H. H. HOPPE, PROF. DR. W. ZELLER
- Tez Türü: Doktora
- Konular: Ziraat, Agriculture
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2001
- Dil: İngilizce
- Üniversite: Georg-August-Universität Göttingen
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 120
Özet
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Özet (Çeviri)
In general, BTH and plant extract treated shoots showed a higher phenol content thanuntreated shoots. This effect was most significant 2-4 d after treatment. Chromatographic(TLC) examinations indicated that some flavonoids (quercetin and kampferol isoforms)increased after treatment with BTH and plant extract. An inhibitory substance was found inBTH treated plants which could, however, not be fully characterized. From the data obtainedthe detected inhibitory substance was assumed to be a phloretin derivate that showedconsiderable reduction of bacterial multiplication in vitro.5.2.2 Enzymatic activities, PR proteins and salicylic acid (SA)Peroxidase activity showed a significant increase in BTH and PE treated shoots from 2?7 dp.i. with the highest activity 4 d after treatment. After SDS-PAGE the main PO activity wasfound in molecular sizes of 20.1 and 43 kD. The plant extract treated shoots showed a highexpression in 3 bands and BTH treated shoots in 2 bands.Treatment with BTH or plant extracts induced an increase of specific protein bands, similarto the pattern after artificial inoculation.Also, phenylalanine-ammonia lyase (PAL) activity was increased in BTH and plant extracttreated shoots. Generally, the activation by PE was significantly higher than by BTHtreatment.Polyphenoloxidase (PPO): Only treatments with plant extracts caused a remarkableenhancement. This result was correlated to high necrotisation in PE treated plants. In contrast,low PPO activity correlated with low necrotization in BTH treated plants. Infection increasedPPO activity much later (11 d p.i.) than PE treatment.ß-Glucosidase activity (ß-Gl) was considerably higher after both treatments, especially 4 dp.i. In BTH treated plants a significant increase of ß-Gl correlated to production of anantimicrobial substance.Glutathione-S-transferase (GST) was strongly increased in BTH treated shoots 4 and 7 d p.i.This result was correlated to low necrotization after BTH treatment in contrast to PEtreatment with more necrotization but less GST activity in the shoots.
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