T cells and cytokines in the Lamina propria of the pig
Başlık çevirisi mevcut değil.
- Tez No: 400600
- Danışmanlar: DR. MICK BAILEY, PROF. CHRIS STOKES
- Tez Türü: Doktora
- Konular: Moleküler Tıp, Veteriner Hekimliği, Molecular Medicine, Veterinary Medicine
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 1997
- Dil: İngilizce
- Üniversite: University of Bristol
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 247
Özet
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Özet (Çeviri)
Accumulating evidence suggests that the intestinal lamina propria may be important in regulating immune responses to environmental antigens. Studies using activated leucocytes from the intestine of normal pigs have suggested cytokine profile biased towards T helper 2 cytokine (interleukin-4, IL-4) compared with systemic T helper 1 cells (interleukin-2, 1L-2). The involvement of T helper 1 cytokines in intestinal damage in humans and mice has led to the suggestion that a local switch to these cytokines may be important in the pathogenesis of post-weaning diarrhoea in pigs. The aims of the work were to determine whether the cytokine profile was dependent on the T cell or the accessory cell environment and whether a switch in cytokine profile occurred following weaning. Methods for purification of porcine T cells were developed and compared. Of the techniques examined, monoclonal anti-CD4 plus magnetic beads (MACS) gave the highest purity and recovery. Modifications to the technique were developed to provide maximum purity. Highly purified CD4' T cells from spleen required CD4' accessory cells (splenic or intestinal) for responses to recall antigen, demonstrating the effectiveness of isolation. However, both splenic or intestinal populations of CD4' cells proliferated in response to mitogen in the absence of accessory cells. Proliferation of splenic, but not intestinal CD41 cells was associated with 1L-2 secretion. Reverse-transcriptase-PCR demonstrated that splenic and intestinal CD4+ cells were the primary source of IL-2 and IL-4, respectively. The results clearly demonstrated that porcine intestinal CD41 T cells are committed to secretion of T helper 2 cytokines and that the accessory cell population had little effect on cytokine production either directly or indirectly. Weaning resulted in transient depression of the ability of systemic T cells to secrete 1L-2. However, cytokine production by intestinal T cells was also depressed. The results demonstrated that simple switching in cytokine profile could not account for the observed damage to intestinal structure, suggesting that the pathogenesis of postweaning diarrhoea may be complex.
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