Identification of astrocytic factors that could play a role in myelination
Başlık çevirisi mevcut değil.
- Tez No: 402972
- Danışmanlar: PROF. SUE BARNETT
- Tez Türü: Doktora
- Konular: Veteriner Hekimliği, Veterinary Medicine
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2015
- Dil: İngilizce
- Üniversite: The University of Glasgow
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 327
Özet
Özet yok.
Özet (Çeviri)
Multiple sclerosis (MS) is generally considered to be an autoimmune disease; it results in areas of focal demyelination known as plaques, where astrocytes are the main constituent and appear hypertrophic and stellate with increased expression of glial fibrillary acidic protein (GFAP). Although these reactive astrocytes can be beneficial for encapsulating areas of tissue necrosis within the glial scar, the pro-inflammatory cytokines and extracellular molecules they secrete are thought to be inhibitors of remyelination. Conversely, astrocytes can secrete anti-inflammatory cytokines in their less activated state and thereby can be beneficial for remyelination; although the mechanisms involved are not clearly understood. We have developed myelinating cultures of embryonic rat mixed spinal cord cells that are plated on neurospherederived astrocyte monolayers, which allow us to manipulate their reactive state and determine any effects of these astrocytes on myelination. To identify any differences in gene expression profiles of several purified astrocyte phenotypes we carried out a microarray analysis using myelinating cultures plated on astrocytes on poly-L-lysine (PLL-astrocytes) or on tenascin C (TnC-astrocytes), untreated or treated with ciliary neurotrophic factor (CNTF) since it was previously shown that CNTF could stimulate myelination in these myelinating cultures and that TnC-astrocytes supported myelination less than PLL-astrocytes. The microarray analysis suggested CCL2, CCL7 and SERPINB2 as potential myelination regulators, which were validated by qRT-PCR but failed to affect axonal ensheathment in myelinating cultures. A metabolomics analysis was also carried out using untreated or CNTF-treated PLL- and TnC-astrocytes. A list of metabolites were associated with different astrocyte phenotypes and highlighted the importance of lipid metabolism, which might be crucial for stimulating myelination. These in vitro astrocyte phenotypes were compared with previously identified in vivo astrocyte reactivity markers, which suggested that PLL- and TnC-astrocytes could reflect in vivo astrocytes to some extent and that TnC-astrocytes might not be as“quiescent”as currently considered. It is possible that TnC-astrocytes suppress myelination by creating an extracellular matrix (ECM) rich environment, while CNTF dissolves the ECM and thus creates a more regenerative environment. Despite similar stimulatory effects of CNTF on mixed neural cells on PLL and TnC, CNTF appears to affect them via different mechanisms showing that distinct reactive astrocytes could react to the same stimulus in different ways as also presented in in vivo studies.
Benzer Tezler
- CDNF ve manf nörotrofik faktörlerinin iskemi sonrası beyin plastisitesi ve onarımı üzerine olan etkileri
Effects of neurotrophic factors cdnf and manf on brain plasticity and repair after brain ischemia
AHMET BURAK ÇAĞLAYAN
Doktora
Türkçe
2017
Fizyolojiİstanbul Medipol ÜniversitesiSinir Bilimi Ana Bilim Dalı
PROF. DR. ERTUĞRUL KILIÇ
- Identification of novel interaction partners of nuclear factor I B
Nükleer faktör I B'nin etkileşim partnerlerinin tanımlanması
AHMET ERDAL YILMAZ
Yüksek Lisans
İngilizce
2013
Biyolojiİstanbul Teknik Üniversitesiİleri Teknolojiler Ana Bilim Dalı
YRD. DOÇ. DR. ASLI KUMBASAR
- Akım sitometri tekniği ile yeni doğan fare beyninden bölgesel olarak izole edilmiş spesifik hücrelerin protein profillerinin incelenmesi
Identification of protein profiles of regionally isolated specific cells from newborn mice brain by flow cytometry
EMRE VATANDAŞLAR
Yüksek Lisans
Türkçe
2017
Biyolojiİstanbul Medipol ÜniversitesiSinir Bilimi Ana Bilim Dalı
YRD. DOÇ. DR. DENİZ DURALI
PROF. DR. ERTUĞRUL KILIÇ
- Novel treatment approaches based on identification of molecular determinants in neuropsychiatric diseases
Başlık çevirisi yok
SENİHA DERYA SARGIN
Doktora
İngilizce
2008
NörolojiGeorg-August-Universität GöttingenNörobilim Ana Bilim Dalı
DR. HANNELORE EHRENREICH
- Miyelin interaktomu: İkili graf tabanlı protein-protein etkileşimli ağlarla multipl skleroz'da hücre-hücre etkileşimlerinin tanımlanması
Myelin interactome: Identification of cell-cellinteractions in multiple sclerosis with binary grafbased protein-protein interaction networks
EŞREF ÇELİK
Yüksek Lisans
Türkçe
2018
Biyolojiİstanbul Medipol ÜniversitesiHistoloji ve Embriyoloji Ana Bilim Dalı
DOÇ. DR. BİLAL ERSEN KERMAN