Geri Dön

DNAJA1 as a novel interacting partner of transglutaminase 2

Başlık çevirisi mevcut değil.

  1. Tez No: 403368
  2. Yazar: ELVAN ERGÜLEN
  3. Danışmanlar: Prof. Dr. LASZLO FESUS
  4. Tez Türü: Doktora
  5. Konular: Mikrobiyoloji, Tıbbi Biyoloji, Microbiology, Medical Biology
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2016
  8. Dil: İngilizce
  9. Üniversite: Debreceni Egyetem
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 65

Özet

Özet yok.

Özet (Çeviri)

Transglutaminase 2 (TG2) is a multifunctional enzyme which participates in posttranslational modification of proteins, such as catalyzing calcium dependent crosslinking of proteins, incorporation of primary amines and deamidation of proteins. In addition, TG2 also acts as a G protein, has protein disulphide isomerase, protein kinase activities and plays non-enzymatic roles such as functioning as an adaptor protein, cell surface adhesion mediator and forming protein scaffolds. In our study, we first compared the transamidation activity and calcium sensitivities of Gly224 and Val224 variants of TG2 and found that the Val variant, which is the actual wild type variant in the human genome, exhibits significantly higher activity and calcium sensitivity compared to Gly variant which was used in many previous studies in other laboratories. In the rest of the project we used Val224“wild type”variant of TG2. Our main aim in this study was to identify the novel interacting partners of human TG2. For this aim, we used NB4 promyelocytic leukemia (APL) cell line which overexpresses TG2 and also other APL related genes upon differentiation by ATRA. Among several other interacting partners, we identified DNAJA1 as a novel interacting partner of TG2. We further comfirmed this interaction via several in vitro experiments such as ELISA and SPR measurements. The same experiments were performed to determine the DNAJA1 binding domain of TG2 and results suggested that core domain of TG2 is the essential domain for TG2-DNAJA1 interaction. Furthermore, DNAJA1 was found to interact mainly with open conformer of TG2. To explore the effect of DNAJA1 on crosslinking activity of TG2 we carried out some in vitro and in situ experiments and observed that DNAJA1 inversely regulates the activity of TG2. Moreover, DNAJA1 was also identified as a novel substrate of TG2. DNAJA1 and TG2 have been independently reported to regulate similar cellular and pathological processes, such as cellular transport, apoptosis, neurodegenerative disorders and cancer. We propose that both the proteins regulate these overlapping functions via intermolecular interactions.

Benzer Tezler

  1. Investigating the effects of PKNOX2 and its interactors in P53 pathway regulation in bone marrow mesenchymal stem cells

    Kemik iliği mezenkimal kök hücrelerinde P53 yolağı regülasyonunda PKNOX2 ve etkileşimlerinin araştırılması

    MUSTAFA KELEŞ

    Yüksek Lisans

    İngilizce

    İngilizce

    2023

    BiyokimyaHacettepe Üniversitesi

    Kök Hücre Ana Bilim Dalı

    PROF. DR. AYŞEN ÖZCAN

  2. Kalıcı atriyal fibrilasyonlu ve sinüs ritimli hastalarda apoptozis ilişkili genlerin karşılaştırmalı analizleri

    Comparative gene expression profile analysis of apoptosis related genes between patients with permanent atrial fibrillation and normal sinus rhythm

    GÜNSELİ ÇUBUKÇUOĞLU DENİZ

    Doktora

    Türkçe

    Türkçe

    2013

    BiyoteknolojiAnkara Üniversitesi

    Temel Biyoteknoloji Ana Bilim Dalı

    PROF. DR. AHMET RÜÇHAN AKAR

  3. Dnaja1 ve dnaja2 genlerinin meme kanserindeki ifadeleri ve genetik/epigenetik alterasyonları

    Expression and genetic/epigenetic alterations of dnaja1 and dnaja2 genes in breast cancer

    FURKAN ÇELEBİ İLERİ

    Yüksek Lisans

    Türkçe

    Türkçe

    2020

    GenetikZonguldak Bülent Ecevit Üniversitesi

    Moleküler Biyoloji Ana Bilim Dalı

    DR. ÖĞR. ÜYESİ TOLGA ACUN