Geri Dön

The role of sphingolipids in cortisol synthesis in the adrenalcortex

Başlık çevirisi mevcut değil.

  1. Tez No: 520483
  2. Yazar: TUBA SELÇUK ÖZBAY
  3. Danışmanlar: Dr. MARION B. SEWER
  4. Tez Türü: Yüksek Lisans
  5. Konular: Biyoloji, Biology
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2005
  8. Dil: İngilizce
  9. Üniversite: Georgia Institute of Technology
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 86

Özet

Özet yok.

Özet (Çeviri)

In the human adrenal cortex, adrenocorticotropin (ACTH) activates steroid hormone biosynthesis by acutely increasing cholesterol delivery to the mitochondria and chronically up-regulating the transcription of steroidogenic genes (including CYP17). Sphingolipids are a diverse family of phospholipids and glycolipids that mediate a wide variety of cellular processes, including apoptosis, proliferation, and survival. Sterol regulatory element binding proteins (SREBPs) are a family of transcription factors that regulate genes that are involved in cholesterol biosynthesis and fatty acid metabolism. In this study, we investigated the role of sphingolipids in ACTH-dependent steroidogenesis. H295R human adrenocortical cells were treated with ACTH or dibutyryl cAMP (Bt2cAMP) for various time periods and the content of several sphingolipid species was quantified by mass spectrometry. Both ACTH and Bt2cAMP decreased cellular amounts of sphingomyelin, ceramides, sphingosine (So) and sphingosine-1-phosphate (S1P). However, both ACTH and Bt2cAMP increased the activity of sphingosine kinase and the amounts of S1P released into the media. Both So and S1P increased CYP17 mRNA expression and increased cortisol biosynthesis. This increase in CYP17 transcription occurs by promoting SREBP binding to an SRE at -450/-436 basepairs upstream of the transcription initiation site. Furthermore, chromatin immunoprecipitation (ChIP) assays revealed that Bt2cAMP and S1P treatment results in an increase in acetylation of histone H3 and SREBP1 binding to CYP17 promoter. Additionally, transient transfection studies using wild type or mutated hCYP17 promoters and RNA interference (RNAi) assays confirmed the role of SREBP1 in mediating the stimulatory effect of S1P on CYP17 transcription. In summary, our studies demonstrate a link between sphingolipid metabolism and ACTH-dependent steroidogenesis which requires the activation of SREBP1 in human adrenal cortex.

Benzer Tezler

  1. Arabidopsis thalina'nın Fusarium oxyporum toksini Fumonisin B1'e karşı savunma cevabında Gigantea (GI) geninin rolünün araştırılması

    Investigation of the role of the Gigentea (GI) gene in the defence response of Arabidopsis thaliana to the Fusarium oxysporum mycotoxin Fumonisin B1

    ZÜLAL AKBA

    Yüksek Lisans

    Türkçe

    Türkçe

    2024

    BiyolojiEge Üniversitesi

    Biyoloji Ana Bilim Dalı

    PROF. DR. AŞKIM HEDİYE ÇETİNEL

  2. Kaveolada β2-AR-Gs-adenilat siklaz kenetinin incelenmesi

    Investigation of β2-AR-Gs adenyly cyclase coupling in caveolae

    ŞÜKRÜ SADIK ÖNER

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2005

    Eczacılık ve FarmakolojiAnkara Üniversitesi

    Farmakoloji Ana Bilim Dalı

    DOÇ. DR. ÖZLEM UĞUR

  3. Defining the effects of genetic loss of cers4/ceramide in the regulation of tumor metastasis via mediating PD-l1 signaling and stress granules

    Cers4/seramid ifade kaybının PD-l1 sinyali ve stres granülleri aracılığı ile tümör metastazının düzenlenmesindeki etkilerin tanımlanması

    VICTORIA ACAR

    Yüksek Lisans

    İngilizce

    İngilizce

    2023

    Moleküler Tıpİstanbul Medipol Üniversitesi

    Moleküler Tıp ve Biyoteknoloji Ana Bilim Dalı

    DOÇ. DR. SALİH GENCER

  4. Investigating lipid signaling in rat hippocampal slices with patch-clamp tight seal whole cell recording technique

    Sıçan hipokampal kesitlerinde lipit sinyal iletiminin tek hücrede sıkı bağlı yama-kenetleme kayıt tekniği kullanılarak araştırılması

    ÖZGÜR GENÇ

    Yüksek Lisans

    İngilizce

    İngilizce

    2007

    BiyofizikBoğaziçi Üniversitesi

    Biyoetik Ana Bilim Dalı

    DOÇ. DR. HALE SAYBAŞILI

  5. Kısa zincirli sfingolipidlerin akciğer kanseri hücreleri üzerindeki etkileri

    Effects of short chain shingolipids on lung cancer cells

    SHAHRİ TAGHİZADE

    Yüksek Lisans

    Türkçe

    Türkçe

    2022

    Eczacılık ve FarmakolojiAnadolu Üniversitesi

    Farmakoloji Ana Bilim Dalı

    DOÇ. DR. GÖKHAN KUŞ