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Androgenetik tütün haploidlerinden dihaploidlerin elde edilmesinde asenaftenden yararlanma

Başlık çevirisi mevcut değil.

  1. Tez No: 6101
  2. Yazar: BETÜL BÜRÜN
  3. Danışmanlar: PROF. DR. ÜLKÜ EMİROĞLU
  4. Tez Türü: Doktora
  5. Konular: Ziraat, Agriculture
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 1988
  8. Dil: Türkçe
  9. Üniversite: Ege Üniversitesi
  10. Enstitü: Fen Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Tarla Bitkileri Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 68

Özet

- 53 - UZET Korabaylar 5 9/

Özet (Çeviri)

- 5k - SUMMARY A tobacco breeding line Karabağlar 59/*+ was grown at Bornova and the anthers uere cultured on Nitsch's (1969) medium. To induce chromosome doubling, asenaphthene treatements uere done at four different stages of culture. Stage I was the stage at which the treatement was applied as soon as the anthers were placed on the medium. Stages II and III were the ends of the U and the B weeks of culture, respectively. The fourth application was performed also at the end of 8 week, but just after the transfer of plantlets from test tubes into culture jars (Stage IV). About 1GD mgs of acenaphthene christals in small gauze bags were hanged into the culture tubes and jars for two and three days. The plantlets which were rooted and grown in jars were brought into the pots and their fertility was contrel- led. Chromosome numbers were determined using UJalther's (1961) method and by studying mitosis with aceto-orcein staining. Besides chromosome counts, sizes of stomata, pollen grains and fertility were considered in distinguish ing diploids. In the control group, majority of the androgenetic plantlets were haploid, but spontaneous diploids (av. 1.09), and few aneuploids were also present. The asenaphthene treatement that was done in culture jars gave the most successful result, average rate of dihaploids being 32,5 and 1+1,3% for two and three day treatements, respectively. On the other hand, among treated plants, besideB haploids and dihaploids, tetraploids, aneuploids, chimeras and mixed-ploids were observed too.

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