Investigating the factors effecting bacteriocins production in the S.Pneumoniae
Başlık çevirisi mevcut değil.
- Tez No: 645617
- Danışmanlar: Belirtilmemiş.
- Tez Türü: Yüksek Lisans
- Konular: Allerji ve İmmünoloji, Endokrinoloji ve Metabolizma Hastalıkları, Allergy and Immunology, Endocrinology and Metabolic Diseases
- Anahtar Kelimeler: S. pneumoniae, bacteriocin, susceptible strain, producer strain, supernatant, environmental factors
- Yıl: 2016
- Dil: İngilizce
- Üniversite: University of Leicester
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 56
Özet
Özet yok.
Özet (Çeviri)
Streptococcus pneumoniae colonises the nasopharynx of healthy individuals and causes lifethreatening diseases. Intraspecies competition is believed to be very important for the predominance of certain strains in nasopharynx which is mediated by the production of bacteriocins (Bogaert et al., 2004; Kochan et al., 2013) It was hypothesized that the environmental conditions such as temperature or the presence of different carbohydrates which are encountered by the pneumococcus during colonisation and invasive disease would have an impact on bacteriocin production (Mereghetti et al., 2008). To test this hypothesis, a collection of pneumococcal strains was tested against each other to identify strains that are capable of producing bacteriocin. Out of 20 strains, seven were found to produce a substance that is found to be inhibitory to the growth of others. In addition, when the supernatants of producer strains were tested against the susceptible overlay strains in broth culture, growth inhibition of overlay strain was detected and this was dose dependent. Once bacteriocin producing strains were identified, the effect of environmental factors including temperature and different sugars on bacteriocin production were evaluated. It was found that nutrient source has a major influence on the inhibitory activity of pneumococcal strains. The knowledge on the factors effecting bacteriocin production would be very valuable to utilise these agents as anti-infective against the pneumococcus.
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