Gümüş nanopartiküllerin mikosentezi: Optimizasyon ve karakterizasyon
Mycosynthesis of silver nanoparticles: Optimization and characterization
- Tez No: 689905
- Danışmanlar: PROF. DR. SEMRA İLHAN
- Tez Türü: Yüksek Lisans
- Konular: Biyoteknoloji, Mikrobiyoloji, Biotechnology, Microbiology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2021
- Dil: Türkçe
- Üniversite: Eskişehir Osmangazi Üniversitesi
- Enstitü: Fen Bilimleri Enstitüsü
- Ana Bilim Dalı: Biyoloji Ana Bilim Dalı
- Bilim Dalı: Temel ve Endüstriyel Mikrobiyoloji Bilim Dalı
- Sayfa Sayısı: 91
Özet
Biocompatible structures are used to eliminate the discomfort caused by microorganisms in the mouth that damage the tissue around the tooth. These structures serve as drug carriers to the inflamed tissue and are called patches. The patches in this study have been prepared by trying and examining different methods and recipes including tea fibre with known benefits for health, highly biocompatible chitosan and polyethylene glycol polymers. The swelling degrees of these biomaterials/patches were determined, the surface structure was examined with scanning electron microscope images, and the alterations of the components in the structure by temperature were investigated with DSC analysis. In order to evaluate biomaterials in regional applications, drug release behaviours were determined by loading amoxicillin, which was selected as a model drug, and its release kinetics were examined. In addition, the effects of the oven and freeze drying methods used during the preparation of the biomaterials on the material properties were also investigated
Özet (Çeviri)
There is increasing interest in biological (green synthesis) approaches due to the need for environmentally friendly and low-cost methods for nanoparticle synthesis. Numerous studies have been conducted using plants, fungi and bacteria in the green synthesis of nanoparticles. Each organism has advantages and disadvantages, but the simplicity of biomass applications with fungi, their intracellular metal absorption and rapid growth, and the production of a wide variety of intracellular and extracellular enzymes make them stand out in nanoparticle biosynthesis. In this study, biosynthesis conditions of silver nanoparticles (AgNP) were optimized by Box-Behnken Experiment design by using Aspergilus flavus, A. clavatus and A. fumigatus biomass isolated from hypersaline medium. The synthesized AgNPs were characterized using UV-vis spectroscopy, Dynamic light scattering (DLS), Zeta potential, Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and. Its antimicrobial activity and antioxidant activity were evaluated by disc diffusion method and DPPH, respectively. Experimental design analysis based on PDI, zeta potential and particle size data showed that the most efficient conditions for AgNP synthesis were 27℃/9pH/0.1mM, 27℃/9pH/0.5mM, and 37℃/9pH/0.3mM for A. flavus. It was determined that 27℃/9pH/0.1mM and 37℃/7pH/0.5mM for A.fumigatus. In measurements made in UV-vis spectroscopy, AgNPs showed characteristic surface plasmon resonance between 420nm and 430nm. AgNPs synthesized by A. flavus and A. fumigatus had spherical morphology ranging from 50-100nm. It was determined that the used test organisms E. coli, S. aureus and C. albicans were sensitive to the synthesized Ag nanoparticles. As a result of the optimization and characterization, it was concluded that fungi can be potential tools in the production of AgNP by green synthesis.
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