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Romatoid artritli hastaların eritrosit ve plazmalarında süperoksit dismutaz ve katalaz aktiviteleri

Superoxide dismutase and catalase activites in erythrocytes and plasma from patients with rheumatoid arthritis

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  1. Tez No: 70364
  2. Yazar: NURAN İŞÇİ
  3. Danışmanlar: DOÇ. DR. ÖMER AKYOL
  4. Tez Türü: Yüksek Lisans
  5. Konular: Biyokimya, Biochemistry
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 1997
  8. Dil: Türkçe
  9. Üniversite: İnönü Üniversitesi
  10. Enstitü: Sağlık Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Biyokimya Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 56

Özet

ÖZET Bu çalışmada tedavi alan (n= 26) ve tedavi almayan (n= 8) rorrçatoid artritli hastaların eritrosit ve plazmalarında antroksidan enzimler olan süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri ölçüldü. SOD aktivitesi ksantin/ksantin oksidaz sistemi ile, katalaz aktivitesi ise H202'in ultraviole spektrum aralığında absorbansının azalması ile tesbit edildi. SOD ölçümünde ayrıca etanol/kloroform (w/w, 5/3) ekstraksiyonu yapılarak görülebilir alandaki ölçümlerde olabilecek artefaktlar önlendi. Bulunan sonuçlar kontrol grubunun (n= 22) enzim aktivite değerleri ile karşılaştırıldı. Plazmada katalaz aktivitesi tesbit edilemedi. Tedavi alan romatoıd artritli hasta grubunda eritrosit SOD aktivitesi 1 184 ± 182 Ü/gr Hb, CAT aktivitesi 66,7 ± 14,6 K/gr Hb bulundu. Bu grupta plazma SOD aktivitesi ise 1,91 ± 1,16 Ü/gr Hb idi. Tedavi almayan romatoid artritli hasta grubunda eritrosit SOD aktivitesi 1220,0 ± 214,6 Ü/gr Hb, CAT aktivitesi 67,4 ± 16,8 K/gr Hb olarak bulundu. Bu grupta plazma SOD aktivitesi 1,35 ± 0.88 Ü/ml (p < 0,05) idi. Kontrol grubunda eritrosit SOD ve CAT aktiviteleri sırasıyla 1 194 ± 172,4 U/gr Hb ve 62,6 ± 15,9 K/gr Hb idi. Ayrıca bu grubun plazma SOD aktivitesi 2,39 ± 0,87 U/mL olarak ölçüldü. Sadece tedavi almayan grubun plazma SOD aktivitesinde istatistiksel olarak anlamlı bir azalma mevcuttu. Sonuçlar literatürün ışığında tartışıldı. Eritrosit içi antioksidan enzim aktivitelerinde kontrole göre istatistiksel açıdan fark bulunmaması, romatoid artritli hastalarda ortaya çıkan aneminin etyolojisinde bu enzimlerin herhangi bir fonksiyonunun olmadığını düşündürdü.7. SUMMARY In the present study, antioxidant enzyme activities such as superoxide dismutase (SOD) and catalase (CAT) were determined in erythrocytes and plasma taken from the patients with rheumatoid arthritis. The patients were divided into two groups; group 1 received the classical treatment (n= 26) and group 2 no treatment for rheumatoid arthritis. SOD activity was determined via xanthine/xanthine oxidase system. CAT activity was determined by the decrease of H2O2 absorbance at ultraviolet spectrum. In the determination of SOD activity, the ethanol/chloroform extraction (w/w, 5/3) was also used to prevent the artefacts originated from the red colour of haemoglobin. The results were compared with enzyme activities of the control group (11= 22). No catalase activity was determined in the plasma taken from both the patient groups and the control groups. Erythrocyte SOD activity was 1 184 ± 182 U/g Hb and CAT activity was 66,7 ± 14,6 K/gr Hb in the patients with rheumatoid arthritis receiving classical treatment (Group I). In group 2, receiving no treatment for rheumatoid arthritis, SOD and CAT activity were 1220,0 ±214,6 U/gr Hb and 67,4 ± 16.8 K/gr Hb respectively. Plasma SOD activity in this group was 1,35 ± 0,88 U/ml (p < 0,05). In control group erythrocyte SOD and CAT activities were 1 194 ± 172.4 U/gr Hb and 62,6 ± 15,9 K/gr Hb respectively and plasma SOD activity was 2,39 ± 0,87 U/mL. As seen, diminishing of plasma SOD activity was significant in the patient group receiving no treatment. The results of the study were discussed in considering literature. As a conclusion, it has suggested that antioxidant enzymes do not play an important role in the etiology of anemia in rheumatoid arthritis, because no significant enzyme activity was determined when compared with control group in our study. 42

Özet (Çeviri)

7. SUMMARY In the present study, antioxidant enzyme activities such as superoxide dismutase (SOD) and catalase (CAT) were determined in erythrocytes and plasma taken from the patients with rheumatoid arthritis. The patients were divided into two groups; group 1 received the classical treatment (n= 26) and group 2 no treatment for rheumatoid arthritis. SOD activity was determined via xanthine/xanthine oxidase system. CAT activity was determined by the decrease of H2O2 absorbance at ultraviolet spectrum. In the determination of SOD activity, the ethanol/chloroform extraction (w/w, 5/3) was also used to prevent the artefacts originated from the red colour of haemoglobin. The results were compared with enzyme activities of the control group (11= 22). No catalase activity was determined in the plasma taken from both the patient groups and the control groups. Erythrocyte SOD activity was 1 184 ± 182 U/g Hb and CAT activity was 66,7 ± 14,6 K/gr Hb in the patients with rheumatoid arthritis receiving classical treatment (Group I). In group 2, receiving no treatment for rheumatoid arthritis, SOD and CAT activity were 1220,0 ±214,6 U/gr Hb and 67,4 ± 16.8 K/gr Hb respectively. Plasma SOD activity in this group was 1,35 ± 0,88 U/ml (p < 0,05). In control group erythrocyte SOD and CAT activities were 1 194 ± 172.4 U/gr Hb and 62,6 ± 15,9 K/gr Hb respectively and plasma SOD activity was 2,39 ± 0,87 U/mL. As seen, diminishing of plasma SOD activity was significant in the patient group receiving no treatment. The results of the study were discussed in considering literature. As a conclusion, it has suggested that antioxidant enzymes do not play an important role in the etiology of anemia in rheumatoid arthritis, because no significant enzyme activity was determined when compared with control group in our study. 428. KAYNAKLAR 1. Slater T.F. Free radicals and tissue injury. Biochem J. 1984; 222: 1-15 2. Kılınç K. Oksijen Radikalleri: Üretilmeleri, Fonksiyonları ve Toksik Etkileri, Biyokimya Dergisi 1985; 10 (2): 60-89. 3 Sies H. Role of reactive oxygen species in biological processes. Clin Wochenschr 1991;69:965-968. 4. Fridovich I. Superoxide dismutases. Ann. Rev. Biochem 1975, 44: 147-159. 5. Fridovich I. Superoxide radical: An endogenous toxicant. Ann Rev. Pharmacol Toxicol. 1983;23:239-57. 6. Aleman V. and Handler P. Dihydroorotate dehydrogenose. The Journal of Biological Chemistry. 1967; 242 (18): 4087-4096. 7. Turrens J.F. and Boveris A. Generation of superoxide anion by the NADH dehydrogenase of bovine heart mitochondria. Biochem J. 1980; 191:421-427. 8. Fi idowich 1. Quantitative aspects of the production of superoxide anion radical by milk xanthine oxidase. The Journal of Biological Chemistry. 1970; 25: 4053- 4057. 9. Sohal R.S., and Brunk U.T. Mitokondrial pruduction of prooxidants and cellular senescense. Mutation Research. 1992; 275: 295-304. lO.Sothorn PA, Powis G. Free radicals in medicine. I. chemical nature and biologic reactions. Mayo Clin Proc. 1988; 63: 381-389. ll.Deby C. and Goutier R. New perspectives on the biochemistry of superoxide anion and the efficiency of superoxide dismutase. Biochemical Pharmacology. 1990;39:399-405. 12Auer D.E., NG CJ. and Seawright A.A. Effect of palosein (superoxide dismutase) and catalase upon oxygen derived free radical induced degradation of equine synavial fluid. Equine Veterinary Journal 1990; 22 (1): 13-17. 13.Britgan BE., Pou S., Rosen G.M., Lilleg D.M. and Buettner G.R. Hydroxyl radical is not a product of the reaction of xanthine oxidase and xanthine. The Journal of Biological Chemistry. 1990; 265 (29): 17533-17538..H

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