مطالعه برهمکنش نانوذرات با سلول انسانی در تراشۀ میکروفلوئیدی
Study of the Interaction of Nanoparticles with Human Cells in MicrofluidicChip
- Tez No: 719809
- Danışmanlar: DR. ZEİNAB BAGHERİ, DR. HAMİDEH EHTESABİ, PROF. DR. HAMİD LATİFİ
- Tez Türü: Yüksek Lisans
- Konular: Biyomühendislik, Genetik, Bioengineering, Genetics
- Anahtar Kelimeler: microfluidic chip, red carbon dots, cancer
- Yıl: 1983
- Dil: Arapça
- Üniversite: Shahid Beheshti University
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 77
Özet
Özet yok.
Özet (Çeviri)
Cancer is the leading cause of death worldwide. The complex and chaotic environment of the tumor makes it very difficult to treat. Two-dimensional cell culture is not ideal for traditional drug screening because twodimensional culture does not accurately determine the physiological conditions of tumor cells. Therefore, it is necessary to use a new cell model that is more similar to the body's cells for the purpose of drug discovery and evaluation. Spheroids are one of the most widely used models of 3D culture that are close to cancerous masses in terms of cell structure, biochemistry and genetics and can be produced in the laboratory using various methods. Among all the methods, microfluidic chips have unique advantages such as small size, possibility of precise control of liquids, reduction of consumables, which has caused them to be considered as an efficient method for spheroid production. In this study, using a 3D printer and soft lithography, a microfluidic chip with wells with a depth of 300 μm was created and it was used as a substrate to study the toxicity of a group of nanomaterials called carbon dot. The carbon dot synthesis used in this study is considered as the second part of this project. The excitation and emission wavelengths of synthesized carbon dot are 456 and 584 nm, respectively, which can be used for bioimaging applications due to its red emission. Visible-ultraviolet absorption spectroscopy, spectrofluorimetry, FTIR, DLS and AFM were used to characterize this carbon dot. The results of MTT test show that different concentrations of this substance did not have significant toxicity on glioblastoma cells and also spheroid formation was performed in the presence of this carbon dot.
Benzer Tezler
- پایاننامه جهت اخذ درجه دکتری تخصصی در رشته بافتشناسی مقایسهایعنوان:مطالعه اثرات مایکوتوکسین سیکلوپیازونیک اسید بر بافتشناسی و آپوپتوز بیضه،خصوصیات و توان باروری اسپرمهای اپیدیدیمی در موش کوچک آزمایشگاهی
Study of the Cyclopiazonic acid effects on testicular histology andapoptosis, epididymal sperm characteristics and its fertility potential inmice
FARZANEH BONYADI
- مهاجرت اجباری از منظر زنان؛ مطالعه موردی زنان افغانستانی وسوری مقیم ایران و ترکیه
Başlık çevirisi yok
ASİYE ŞİMŞEK ADEMİ
- شناخت و تحلیل طبقه بندی های موجود طرح و نقش قالی ایران
Analysis and Cognition of exciting classification Designs andMotifs of Persian rugs
SIAMAK EGHARLOO
- أسلوب الكناية في الجزءين التاسع والعشرين والثلاثين من القران الكريم
Üslubu l- kinayefil- cüzeyn salâinmine l- Kur âni l- Kerîm (retorik analitik çalışma)
SALIM SABAH OTHMAN SHWAN
Yüksek Lisans
Arapça
2021
DinTokat Gaziosmanpaşa ÜniversitesiTemel İslam Bilimleri Ana Bilim Dalı
DR. ÖĞR. ÜYESİ İBRAHİM İBRAHİMOĞLU
