Antibacterial activity of hypericumperforatum extracts againstescherichia coli, bacillus subtilis,clinical isolates of methicillinresistance- and multi drugresistance staphylococcus aureus
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- Tez No: 719831
- Danışmanlar: DR. MUKHLESUR RAHMAN
- Tez Türü: Yüksek Lisans
- Konular: Biyomühendislik, Tıbbi Biyoloji, Bioengineering, Medical Biology
- Anahtar Kelimeler: H. perforatum, extracts, B. subtilis, E. coli, MRSA strains, TLC, UPLC
- Yıl: 2022
- Dil: İngilizce
- Üniversite: University of East London
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 36
Özet
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Özet (Çeviri)
Hypericum perforatum, also known as St. John's Wort, has been used for centuries as a herbal remedy against variety of diseases for its biological activities such as antidepressant, antioxidant, and antitumor activity. It has been also used as an antibacterial agent against different bacteria for decades. The aim of this study is to investigate the antibacterial property of the H. perforatum extracts against several bacterial strains and determine its chemical composition. Plant material was extracted with Soxhlet extraction technique with 3 different solvents, including n-hexane, dichloromethane and methanol, respectively. The minimum inhibitory concentration assay was done against several S. aureus strains including standard strain ATCC 25923, MRSA strains including XU212, and SA 1199B, and also B. subtilis, and E. coli. Antibacterial activity was determined in comparison with the positive control, which is Norfloxacin. In terms of S. aureus strains, n-hexane extract demonstrated the best activity against XU212 strain, which is the tetracycline-resistant strain as it possesses tetK efflux pump, with the MIC of 256 µg/mL. The MIC was same and 512 µg/mL for the rest of the S. aureus strains for n-hexane and dichloromethane extracts. n-hexane extract demonstrated a promising inhibitory activity against E. coli ATCC 25922 with the MIC of 1 µg/mL, which was the lowest concentration tested. Finally, MIC of nhexane and dichloromethane extracts were ranging between 64 – 128 µg/mL and 256 – 512 µg/mL, respectively, against B. subtilis. Methanol extract didn't demonstrate activity against any of the tested bacteria. UPLC was performed for dichloromethane and methanol extracts with a mobile phase of acetonitrile and water. Main compounds were identified as Catechin, Hyperforin, and Rutin for methanol extract, and Hypericin and Luteoskyrin for dichloromethane extract.
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