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Investigation on the effect of adult mouse kidney extract on differentiation of human embryonic stem cells to kidney precursor cells

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  1. Tez No: 837209
  2. Yazar: MARYAM AHMAD ZADEH
  3. Danışmanlar: Belirtilmemiş.
  4. Tez Türü: Yüksek Lisans
  5. Konular: Biyoloji, Biology
  6. Anahtar Kelimeler: 2D culture, 3D culture, embryonic stem cells, suspension, spectrophotometer, cDNA
  7. Yıl: 2019
  8. Dil: Arapça
  9. Üniversite: Islamic Azad University
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 95

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Özet (Çeviri)

Background: The aim of this study was to investigate the effect of adult mouse kidney extracts on the differentiation of human embryonic stem cells into kidney precursor cells at three concentrations of 25, 12.5 and 50% in 2D and 3D Culture medium. The embryonic stem cells are multiplicative potential cells derived from the internal cell wall of the embryonic blastocyst stage. Previous studies have shown that fetal cells are capable of producing renal mesenchymal and differentiating into nephron, under laboratory conditions. Materials and Methods: This was an experimental study. Initially, the human plutonium stem cell culture medium was enriched. The required fibroblasts were then prepared from the human foreskin. Then the fibroblastic cells were passed and multiplied. In this design, human embryonic stem cells (Royan H5) were used. Cells were initially cultured on an extracellular matrix (sigma-Aldarich, E1270 ECM;) in a 2-dimensional manner to reach the required amount of research groups. Human pluripotent stem cells were then cultured on the extracellular matrix. Subsequently cultured human stem cells cultured and single human pluripotent stem cells were cultured. The accumulation of human plutonium stem cells was procassed. Real-time RT-PCR reaction and extraction of RNA from human high-powered stem cells. The extracted RNA was quantitated by spectrophotometer. Then cDNA synthesis took place. Real time PCR and quantitative evaluation of genes expression. Finally, the results were analyzed statistically. Results: The results showed that the expression of markers such as E-cadherin, CD24, CD133, PAX, WT1, ZO1 in cultivars of most markers and in both 2D and 3D environments increased the meaningful difference and meaning difference You have been compared to control samples. Conclusion: Due to the results of the expression of human embryonic stem cell markers, they can differentiate into renal precursor cells by the effect of the kidney extract of adult mice.

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