Geri Dön

Expression of selected mirnas (micrornas) in ovine mammary epithelial cells

Başlık çevirisi mevcut değil.

PDF İndir

  1. Tez No: 883902
  2. Yazar: ABDIAZIZ NUR IBRAHIM
  3. Danışmanlar: Prof. Dr. MEHMET ULAŞ ÇINAR
  4. Tez Türü: Yüksek Lisans
  5. Konular: Biyoteknoloji, Ziraat, Biotechnology, Agriculture
  6. Anahtar Kelimeler: Mastitis disease, miRNA expression, Akkaraman sheep, Mammary gland, Mastitis disease, miRNA expression, Akkaraman sheep, Mammary gland
  7. Yıl: 2024
  8. Dil: İngilizce
  9. Üniversite: Erciyes Üniversitesi
  10. Enstitü: Fen Bilimleri Enstitüsü
  11. Ana Bilim Dalı: Tarım Bilimleri ve Teknolojileri Ana Bilim Dalı
  12. Bilim Dalı: Zootekni Bilim Dalı
  13. Sayfa Sayısı: 72

Özet

Mastitis is an inflammation of the mammary glands in sheep. The main causative agent of mastitis in livestock is the gram-positive bacterium, Staphylococcus aureus. MicroRNAs (miRNAs) are small, single-stranded, endogenous, non-coding RNA molecules that consist of 18-26 nucleotides. Diverse miRNA expression patterns and the abundance of potential miRNA targets suggest that miRNAs are likely to be involved in various biological processes, including cell growth, cellular differentiation, proliferation, development, metabolism, immune response to foreign bodies and disease. This study focuses on the expression of these selected miRNAs, miR-148a, and miR-106b in ovine mammary epithelial cells. The primary objectives are to investigate the expression patterns of these miRNAs in ovine mammary epithelial cells and to explore their putative miRNA indicators for selecting sheep against mastitis disease, a prevalent and immunologically significant disease in dairy sheep. To this study, ovine mammary epithelial cells were isolated from a local Turkish breed (Akkaraman) cultured and stimulated with S. aureus, then total RNA was extracted, and cDNA was synthesized under protocol conditions. Quantitative real-time PCR (qRT-PCR) was utilized to measure the expression levels of miR-148a and miR-106b by using U6 snRNA as a housekeeping gene. Our analysis indicates that the target genes miR-106b and miR-148a are involved in important immune processes, including cytokine signaling, inflammatory responses, and immune cell activation. The qRT-PCR results showed that the miR-106b and miR-148a were differentially expressed between the control and stimulated groups. The miR-106b was downregulated while miR-148a was upregulated. Target gene analysis was performed by using TargetScan database and identified 642 & 3747 potential target genes of miR-106b and miR-148a, respectively. Gene Ontology (GO) annotation was carried out by using geneXplain tool. Therefore these miRNAs play a role in regulating immune responses and inflammation during mastitis, ultimately contributing to a deeper understanding of the mechanisms underlying ovine mastitis disease. In conclusion, this study bridges molecular insights with practical applications, revolutionizing mastitis management through miRNA-mediated mechanisms and advanced breeding practices.

Özet (Çeviri)

Mastitis is an inflammation of the mammary glands in sheep. The main causative agent of mastitis in livestock is the gram-positive bacterium, Staphylococcus aureus. MicroRNAs (miRNAs) are small, single-stranded, endogenous, non-coding RNA molecules that consist of 18-26 nucleotides. Diverse miRNA expression patterns and the abundance of potential miRNA targets suggest that miRNAs are likely to be involved in various biological processes, including cell growth, cellular differentiation, proliferation, development, metabolism, immune response to foreign bodies and disease. This study focuses on the expression of these selected miRNAs, miR-148a, and miR-106b in ovine mammary epithelial cells. The primary objectives are to investigate the expression patterns of these miRNAs in ovine mammary epithelial cells and to explore their putative miRNA indicators for selecting sheep against mastitis disease, a prevalent and immunologically significant disease in dairy sheep. To this study, ovine mammary epithelial cells were isolated from a local Turkish breed (Akkaraman) cultured and stimulated with S. aureus, then total RNA was extracted, and cDNA was synthesized under protocol conditions. Quantitative real-time PCR (qRT-PCR) was utilized to measure the expression levels of miR-148a and miR-106b by using U6 snRNA as a housekeeping gene. Our analysis indicates that the target genes miR-106b and miR-148a are involved in important immune processes, including cytokine signaling, inflammatory responses, and immune cell activation. The qRT-PCR results showed that the miR-106b and miR-148a were differentially expressed between the control and stimulated groups. The miR-106b was downregulated while miR-148a was upregulated. Target gene analysis was performed by using TargetScan database and identified 642 & 3747 potential target genes of miR-106b and miR-148a, respectively. Gene Ontology (GO) annotation was carried out by using geneXplain tool. Therefore these miRNAs play a role in regulating immune responses and inflammation during mastitis, ultimately contributing to a deeper understanding of the mechanisms underlying ovine mastitis disease. In conclusion, this study bridges molecular insights with practical applications, revolutionizing mastitis management through miRNA-mediated mechanisms and advanced breeding practices.

Benzer Tezler

  1. Tez No

    177556

    Investigation of microRNA's on genomic instability regions in breast cancer

    Meme kanserinde genomik instabilite bölgelerindeki mikroRNA?ların araştırılması

    ŞADAN DUYGU SELÇUKLU

    Yüksek Lisans

    İngilizce

    İngilizce

    2007

    BiyolojiOrta Doğu Teknik Üniversitesi

    Biyoloji Ana Bilim Dalı

    DOÇ.DR. CENGİZ YAKICIER

    Y.DOÇ.DR. AYŞE ELİF ERSON

  2. Tez No

    781373

    Sıçan pankreatik beta hücre hattında (INS-1E) lipotoksisiteye verilen cevapta fonksiyon gösteren parkin proteininin regülasyonunda rol alan mikroRNA'ların saptanması

    Detection of microRNAs in the regulation of parkin protein functioning in the response to lipotoxicity in rat pancreatic beta cell line (INS-1E)

    HATİCE PINAR BAYSAN ÇEBİ

    Doktora

    Türkçe

    Türkçe

    2023

    BiyoteknolojiAnkara Üniversitesi

    Temel Biyoteknoloji Ana Bilim Dalı

    DOÇ. DR. BALA GÜR DEDEOĞLU

  3. Tez No

    809398

    MİR-182, MİR-320a, MİR-144'ün ebv-pozitif burkıtt lenfoma ile olan ilişkisinin araştırılması

    Analysis of the association of MİR-182, MİR-320a, MİR-144 with EBV-positive burkitt lymphoma

    MEVSİM SAYDAM

    Yüksek Lisans

    Türkçe

    Türkçe

    2023

    GenetikOndokuz Mayıs Üniversitesi

    Moleküler Tıp Ana Bilim Dalı

    DOÇ. DR. SERCAN ERGÜN

    PROF. DR. AYHAN DAĞDEMİR

  4. Tez No

    471412

    Probiyotiklerin insan miRNA'ları üzerine etkileri

    Effects of probiotics on the human miRNAs

    MERVE İLHAN

    Yüksek Lisans

    Türkçe

    Türkçe

    2017

    BiyokimyaNecmettin Erbakan Üniversitesi

    Tıbbi Biyokimya Ana Bilim Dalı

    PROF. DR. MEHMET GÜRBİLEK

  5. Tez No

    567433

    Tekrarlayan implantasyon başarısızlığında, doku ve dolaşımdaki MikroRNA'ların etkisi

    The role of the serum exosomal and endometrium MicroRNAs in recurrent implantation failure

    FATEMEH AZHARI ZARNAGH

    Doktora

    Türkçe

    Türkçe

    2019

    Moleküler Tıpİstanbul Üniversitesi

    Moleküler Tıp Ana Bilim Dalı

    PROF. DR. SADRETTİN PENÇE

Geri Dön