Expression of selected mirnas (micrornas) in ovine mammary epithelial cells
Başlık çevirisi mevcut değil.
- Tez No: 883902
- Danışmanlar: Prof. Dr. MEHMET ULAŞ ÇINAR
- Tez Türü: Yüksek Lisans
- Konular: Biyoteknoloji, Ziraat, Biotechnology, Agriculture
- Anahtar Kelimeler: Mastitis disease, miRNA expression, Akkaraman sheep, Mammary gland, Mastitis disease, miRNA expression, Akkaraman sheep, Mammary gland
- Yıl: 2024
- Dil: İngilizce
- Üniversite: Erciyes Üniversitesi
- Enstitü: Fen Bilimleri Enstitüsü
- Ana Bilim Dalı: Tarım Bilimleri ve Teknolojileri Ana Bilim Dalı
- Bilim Dalı: Zootekni Bilim Dalı
- Sayfa Sayısı: 72
Özet
Mastitis is an inflammation of the mammary glands in sheep. The main causative agent of mastitis in livestock is the gram-positive bacterium, Staphylococcus aureus. MicroRNAs (miRNAs) are small, single-stranded, endogenous, non-coding RNA molecules that consist of 18-26 nucleotides. Diverse miRNA expression patterns and the abundance of potential miRNA targets suggest that miRNAs are likely to be involved in various biological processes, including cell growth, cellular differentiation, proliferation, development, metabolism, immune response to foreign bodies and disease. This study focuses on the expression of these selected miRNAs, miR-148a, and miR-106b in ovine mammary epithelial cells. The primary objectives are to investigate the expression patterns of these miRNAs in ovine mammary epithelial cells and to explore their putative miRNA indicators for selecting sheep against mastitis disease, a prevalent and immunologically significant disease in dairy sheep. To this study, ovine mammary epithelial cells were isolated from a local Turkish breed (Akkaraman) cultured and stimulated with S. aureus, then total RNA was extracted, and cDNA was synthesized under protocol conditions. Quantitative real-time PCR (qRT-PCR) was utilized to measure the expression levels of miR-148a and miR-106b by using U6 snRNA as a housekeeping gene. Our analysis indicates that the target genes miR-106b and miR-148a are involved in important immune processes, including cytokine signaling, inflammatory responses, and immune cell activation. The qRT-PCR results showed that the miR-106b and miR-148a were differentially expressed between the control and stimulated groups. The miR-106b was downregulated while miR-148a was upregulated. Target gene analysis was performed by using TargetScan database and identified 642 & 3747 potential target genes of miR-106b and miR-148a, respectively. Gene Ontology (GO) annotation was carried out by using geneXplain tool. Therefore these miRNAs play a role in regulating immune responses and inflammation during mastitis, ultimately contributing to a deeper understanding of the mechanisms underlying ovine mastitis disease. In conclusion, this study bridges molecular insights with practical applications, revolutionizing mastitis management through miRNA-mediated mechanisms and advanced breeding practices.
Özet (Çeviri)
Mastitis is an inflammation of the mammary glands in sheep. The main causative agent of mastitis in livestock is the gram-positive bacterium, Staphylococcus aureus. MicroRNAs (miRNAs) are small, single-stranded, endogenous, non-coding RNA molecules that consist of 18-26 nucleotides. Diverse miRNA expression patterns and the abundance of potential miRNA targets suggest that miRNAs are likely to be involved in various biological processes, including cell growth, cellular differentiation, proliferation, development, metabolism, immune response to foreign bodies and disease. This study focuses on the expression of these selected miRNAs, miR-148a, and miR-106b in ovine mammary epithelial cells. The primary objectives are to investigate the expression patterns of these miRNAs in ovine mammary epithelial cells and to explore their putative miRNA indicators for selecting sheep against mastitis disease, a prevalent and immunologically significant disease in dairy sheep. To this study, ovine mammary epithelial cells were isolated from a local Turkish breed (Akkaraman) cultured and stimulated with S. aureus, then total RNA was extracted, and cDNA was synthesized under protocol conditions. Quantitative real-time PCR (qRT-PCR) was utilized to measure the expression levels of miR-148a and miR-106b by using U6 snRNA as a housekeeping gene. Our analysis indicates that the target genes miR-106b and miR-148a are involved in important immune processes, including cytokine signaling, inflammatory responses, and immune cell activation. The qRT-PCR results showed that the miR-106b and miR-148a were differentially expressed between the control and stimulated groups. The miR-106b was downregulated while miR-148a was upregulated. Target gene analysis was performed by using TargetScan database and identified 642 & 3747 potential target genes of miR-106b and miR-148a, respectively. Gene Ontology (GO) annotation was carried out by using geneXplain tool. Therefore these miRNAs play a role in regulating immune responses and inflammation during mastitis, ultimately contributing to a deeper understanding of the mechanisms underlying ovine mastitis disease. In conclusion, this study bridges molecular insights with practical applications, revolutionizing mastitis management through miRNA-mediated mechanisms and advanced breeding practices.
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