Synthesis and characterization of boronic acid functionalized sorbents for the antibody removal from human plasma
İnsan plazmasından antibadilerin uzaklaştırılması için boronik asit fonksiyonu içeren destek malzemelerinin hazırlanması ve karakterizasyonu
- Tez No: 216381
- Danışmanlar: PROF. DR. ADİL DENİZLİ
- Tez Türü: Yüksek Lisans
- Konular: Kimya, Chemistry
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2007
- Dil: İngilizce
- Üniversite: Hacettepe Üniversitesi
- Enstitü: Fen Bilimleri Enstitüsü
- Ana Bilim Dalı: Kimya Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 67
Özet
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Özet (Çeviri)
In this study a novel and new approach was developed to obtain an efficient separation of human-IgG from human plasma. 4-Vinylphenylboronic acid (VPBA) and 2-hydroxyethylmethacrylate (HEMA) were chosen as a monomer and co-monomer, respectively. Spherical microbeads with an average size of 100-140 µm were obtained by the radical suspension polymerization of VPBA and HEMA conducted in an aqueous dispersion medium. VPBA monomer was characterized by FTIR. p(HEMA-VPBA) beads were characterized by swelling studies, FTIR. The pHEMA and p(HEMA-VPBA) microbeads were contacted with blood in in-vitro systems. Loss of the blood cells and the clotting times were also followed. Good blood compatibility properties of both pHEMA and p(HEMA-VPBA) were clearly observed with blood coagulation experiments. Loss of cells in the blood contacting with pHEMA and p(HEMA-VPBA) microbeads were nearly the same. These VPBA containing p(HEMAVPBA) microbeads with a swelling ratio of 57 % were used in the adsorption/desorption of human-IgG from aqueous solutions and human plasma. The maximum human-IgG adsorption (139 mg/g polymer) was observed at pH 8.5 for HEPES buffer. The data obtained from the adsorption of human plasma shows that the affinity microbeads can not be used for the one-step separation of human-IgG from human plasma. It was also observed that human-IgG could be repeatedly adsorbed and desorbed with p(HEMA-VPBA) microbeads without significant loss in the adsorption capacity. Key Words : Boronate Affinity Chromatography, Boronic Acid, Glycoproteins, Suspension polymerization, Affinity binding
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