Geri Dön

Dissecting the role of estrogen receptor palmitoylation in breast cancer cells

Başlık çevirisi mevcut değil.

  1. Tez No: 402068
  2. Yazar: ŞERİFE AYAZ GÜNER
  3. Danışmanlar: DR. WEI XU
  4. Tez Türü: Doktora
  5. Konular: Göğüs Kalp ve Damar Cerrahisi, Radyasyon Onkolojisi, Radyoloji ve Nükleer Tıp, Thoracic and Cardiovascular Surgery, Radiation Oncology, Radiology and Nuclear Medicine
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2013
  8. Dil: İngilizce
  9. Üniversite: University of Wisconsin-Madison
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 169

Özet

Özet yok.

Özet (Çeviri)

Estrogen signaling is primarily mediated by two estrogen receptors (ERs), ERα and ERβ. ERα is expressed in ~70% of breast cancers and is an important diagnostic and therapeutic target. Developing better treatment options and overcoming limitations of endocrine therapy depend on a detailed understanding of ERα-signaling pathways. ERα, a member of the class I nuclear receptor superfamily of transcription factors, localizes mainly to the nucleus and interacts with DNA regulatory sequences either directly or through interaction with other transcription factors to regulate gene transcription. ERα is also rapidly activates signaling cascades. S-palmitoylation, a reversible lipid modification is catalyzed by palmitoyl acyl-transferases (PAT), which increase affinity of proteins to the membrane. Based on the results of previous studies, it is hypothesized that palmitoylation of ERα regulates extranuclear and nuclear signaling of ERα. We utilized palmitoylation-defective mutant ERαC447A-expressing MDA-MB-468 breast cancer cells to dissect the role of palmitoylation in a breast cancer cell line model. The substitution of ER palmitoylation site abrogated ERα palmitoylation,membrane localization and estrogen-dependent phosphorylation of ERK1/2 in MDAMB- 468 cell line. Besides loss of E2-dependent extranuclear signaling, the substitution of palmitoylation sites led to the loss of other ERα-dependent events in ERC447A expressing MDA-MB-468 cells, such as decreased E2-dependent S118 phosphorylation, impaired regulation of certain target genes, and loss of estrogendependent cell cycle inhibition. This study thus highlights the importance of ERα palmitoylation in both nuclear and extranuclear ER signaling pathways in breast cancer cells. A better understanding of the mechanisms of estrogen action will help us to design more effective drugs affecting signal pathways depending on both membrane and nuclear receptors.

Benzer Tezler

  1. Initial characterization of CXXC5 as a putative DNA binding protein

    Potensiyel olarak DNA'ya bağlanan CXXC5 proteininin ön karakterizasyonu

    PELİN YAŞAR

    Yüksek Lisans

    İngilizce

    İngilizce

    2015

    BiyolojiOrta Doğu Teknik Üniversitesi

    Biyoloji Ana Bilim Dalı

    DOÇ. DR. MESUT MUYAN

  2. Senkron saptanan aksiller lenf nodu metastazında patolojik diskordans var mı?

    Is there pathological discordance in metastasis of sentinel node-detected axillary lymph nodes?

    BERKAY DEMİRÖRS

    Tıpta Uzmanlık

    Türkçe

    Türkçe

    2024

    Genel CerrahiSağlık Bilimleri Üniversitesi

    Genel Cerrahi Ana Bilim Dalı

    DR. MEHMET EMRAH BAYAM

  3. Deneysel epilepsi modelinde içeri doğrultucu potasyum kanallarının kalpteki rolü

    The role of cardiac inwardly rectifying potassium channels in the experimental epilepsy model

    ENES AKYÜZ

    Doktora

    Türkçe

    Türkçe

    2017

    BiyofizikMarmara Üniversitesi

    Biyofizik Ana Bilim Dalı

    DOÇ. DR. PINAR MEGA TİBER

  4. The theoretical formation of Mise en Abyme and its intertextual applicability in Michael Cunningham's The Hours

    Mise en Abyme'in teorik oluşumu ve Michael Cunningham'ın Saatler adlı eserinde metinlerarası ortaya çıkışı

    EGE İBRAHİM MÜLAYİM

    Yüksek Lisans

    İngilizce

    İngilizce

    2023

    İngiliz Dili ve EdebiyatıTekirdağ Namık Kemal Üniversitesi

    İngiliz Dili ve Edebiyatı Ana Bilim Dalı

    PROF. DR. TATIANA GOLBAN