Geri Dön

Stage-specific binding profile of cohesin in resting and switch-activated B lymphocytes

Başlık çevirisi mevcut değil.

PDF İndir

  1. Tez No: 402648
  2. Yazar: GAMZE GÜNAL
  3. Danışmanlar: PROF. DR. ROLF JESSBERGER
  4. Tez Türü: Doktora
  5. Konular: Biyomühendislik, Bioengineering
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2013
  8. Dil: İngilizce
  9. Üniversite: Technısche Unıversıtat Dresden
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: Belirtilmemiş.

Özet

Özet yok.

Özet (Çeviri)

Cohesin is required for cell proliferation as it facilitates sister chromatid cohesion in dividing cells. Other regulatory functions of cohesin involve DNA repair, DNA recombination and transcriptional regulation. Cohesin also modulates antigen receptor gene rearrangements by facilitating higher-order chromatin interactions through various chromatin-associated factors. The surface expression of secondary immunoglobulin isotypes in B cells require the activation of essential transcription programs and class switch recombination by where the donor and acceptor S regions at the Igh locus are juxtaposed and the intervening DNA is irreversibly excised. This work aimed at elucidating the potential role of cohesin in class switch recombination and mature B cell specific gene expression, and regulation of chromatin at the Igh locus via the analysis of stage-specific binding profile of cohesin to chromatin in resting and switch-activated B lymphocytes. The first part of this study describes the significant, stage-dependent changes in cohesin binding to the B cell genome as well as its specific interactions during CSR- and at B-cell-related gene loci. In addition, the cohesin binding profile was compared to publicly available ChIP-seq profiles of the transcription regulator CTCF, and the transcription factor EBF1 in pro- and/or mature B cells. Cohesin and CTCF colocalize to the Igh locus in a stage-specific manner. EBF1, CTCF and cohesin are all found at the 3 Igh regulatory region. The finding of these potential regulatory sites will provide a concrete basis for the identification of new regulatory chromatin interactions. Moreover, cohesin is shown to be underrepresented at switch regions. Due to the lack of cohesin at S regions, facilitation of S/S synapsis by cohesin is unlikely. Cohesin underrepresentation at S regions suggest that S regions possess a cohesin-free structure to facilitate accessibility for AID and switch recombination machinery. The second part of this study aimed at establishing a mouse model for cohesin deficiency to study the potential role of cohesin in cells that undergo class switching. Cre transduced Smc3fl=fl B cells show reduced numbers of IgG1+ cells in vitro, due to increased apoptosis following SMC3-knockdown. A cohesin hypomorph phenotype could not be accomplished in animals with heterozygous mutations, since there was no difference in Smc3 mRNA and protein levels. Mice carrying the homozygous mutant were not viable. In conclusion, cohesin binds differentially to B-cell specific gene loci and to the Igh locus upon induction for class switching. These data suggest pathways by which gene expression is controlled, during the transition of IgM+ B cells into secondary Ig-secreting plasma cells, through the regulation of chromatin by cohesin.

Benzer Tezler

  1. Tez No

    624681

    Erişkin kedi ovaryumunda pluripotent kök hücre profili

    The profile of the pluripotent stem cells in adult domestic cat

    ERGÜL ERGEN

    Yüksek Lisans

    Türkçe

    Türkçe

    2017

    Histoloji ve EmbriyolojiErciyes Üniversitesi

    Veterinerlik Histoloji ve Embriyoloji Ana Bilim Dalı

    PROF. DR. NARİN LİMAN

  2. Tez No

    885007

    Investigation of n-terminus mll complexes on the reversion of taxane resistance in castration-resistant prostate cancer

    N-terminal mll komplekslerinin kastrasyona dirençli prostat kanserinde taksan direncini geri döndürmedeki rollerinin araştırılması

    İPEK BULUT

    Doktora

    İngilizce

    İngilizce

    2024

    Moleküler TıpKoç Üniversitesi

    Tıbbi Genetik Ana Bilim Dalı

    PROF. DR. CEYDA AÇILAN AYHAN

  3. Tez No

    518083

    Examination of the correlation between Helicobacter pylori and T cell response with the determination of PD-1 expression level in gastric pathologies

    Mide patolojilerinde PD-1 ekspresyon seviyesinin tayini ile Helikobakter pylori ve T hücre cevabı arasındaki korelasyonun incelenmesi

    TEVRİZ DİLAN DEMİR

    Yüksek Lisans

    İngilizce

    İngilizce

    2018

    Tıbbi Biyolojiİstanbul Teknik Üniversitesi

    Moleküler Biyoloji-Genetik ve Biyoteknoloji Ana Bilim Dalı (disiplinlerarası)

    DOÇ. DR. AYÇA SAYI YAZGAN

  4. Tez No

    896644

    Yeni bazı pirimidin nükleobaz ve nükleozit analoğu bileşiklerin sentez, yapı aydınlatması ve over kanser hücrelerinde sitotoksik aktiviteleri üzerinde çalışmalar

    Studies on the synthesis, structure elucidation and cytotoxic activities of some new pyrimidine nucleobase and nucleoside analogue compounds in ovarian cancer cells

    HİLAL ZIVALI

    Yüksek Lisans

    Türkçe

    Türkçe

    2024

    Eczacılık ve FarmakolojiAnkara Üniversitesi

    Farmasötik Kimya Ana Bilim Dalı

    PROF. DR. MERAL TUNÇBİLEK

  5. Tez No

    993566

    Sülfonat birimleri içeren bazı yeni hidrazon türevlerinin sentezi ve biyolojik aktivitelerinin araştırılması

    Synthesis of some new hydrazone derivatives containing sulfonate units and and investigation of their biological activities

    FATİH DEDEOĞLU

    Yüksek Lisans

    Türkçe

    Türkçe

    2025

    KimyaBatman Üniversitesi

    Kimya Ana Bilim Dalı

    DR. ÖĞR. ÜYESİ MURAT SUNKUR

    DOÇ. DR. AYKUT ÖZTEKİN

Geri Dön