Differential laser-induced perturbation spectroscopy for analysis of biological and bio-related materials
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- Tez No: 402720
- Danışmanlar: PROF. DAVID W. HAHN
- Tez Türü: Doktora
- Konular: Biyofizik, Biyomühendislik, Biyoteknoloji, Biophysics, Bioengineering, Biotechnology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2016
- Dil: İngilizce
- Üniversite: University of Florida
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 135
Özet
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Özet (Çeviri)
Laser-based diagnostic tools have been under research since the use of lasers in clinical medicine, with the main target to find common reliable tools for early diagnosis of degenerative diseases such as cancer. For this reason, a novel laser-based optical sensing scheme, differential-laser induced perturbation spectroscopy (DLIPS), is developed which combines common spectroscopic methods such as Raman spectroscopy and fluorescence spectroscopy with UV laser perturbation via difference spectroscopy. Ultraviolet (UV) laser perturbation is used in this work at very low intensities to induce permanent photochemistry. The novel technique has high potential in detection of abnormalities in tissue in early stages. Additionally, the new method is expected to be a popularly used tool with less patient-to-patient variation and having higher sensitivity and specificity rather than traditional spectroscopic schemes. The research has been designed to develop and maximize the performance of the DLIPS method, and eventually enable it to be used in applications in pathology, including in vivo tissue analysis. The work herein covers the continuation of fundamental research on DLIPS and newer steps to advance its performance on cancer detection to further clinical applications. Three main studies will be presented including DLIPS realized with a Raman probe, DLIPS realized with a fluorescence probe, and DLIPS used to detect cancer from human skin tissues in comparison with a fluorescence probe. The DLIPS is realized with the Raman probe in situ for the first time and its performance is evaluated by classifying the proteins and dipeptides, L-Alanine, Glycine, L-Proline, Ala-Gly, Gly-Gly, Gly-Pro, which are the basic building blocks of biological molecules. Accordingly, a 40% improvement on classification is observed in these Raman studies. The DLIPS is realized with fluorescence probe by exciting the endogenous fluorophore, L-Phenylalanine, L-Tyrosine and L-Tryptophan, mixtures with 193 nm, and perturbing them with 193, 220, 230, and 245 nm wavelengths. Overall a 20% improvement on classification is observed in the fluorescence studies. Finally, DLIPS is compared with a fluorescence probe by exciting/perturbing skin samples with 193 nm. The application was successful in yielding differences in spectral features, but quantitatively did not outperform fluorescence probe for tissue classification.
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