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Detection of foodborne bacterial pathogens using surface enhanced Raman scattering immunoassays

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  1. Tez No: 402808
  2. Yazar: ÖZLEM IŞIK
  3. Danışmanlar: DR. KAREN FAULDS
  4. Tez Türü: Yüksek Lisans
  5. Konular: Bilim ve Teknoloji, Biyomühendislik, Biyoteknoloji, Science and Technology, Bioengineering, Biotechnology
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2015
  8. Dil: İngilizce
  9. Üniversite: University of Strathclyde
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Belirtilmemiş.
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 50

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Özet (Çeviri)

This thesis involves the design of a biosensor which enables a rapid, highly sensitive and selective detection of foodborne pathogens using surface enhanced Raman Scattering (SERS). To generate this detection assay, the specific interaction between an antibody and its corresponding pathogen bacteria was employed. In addition, combination of antibodies with silver nanoparticles and silver coated magnetic nanoparticles was used to obtain a SERS signal. The likeliness of a foodborne outbreak still remains an inevitable threat for public health in both developed and developing countries despite the advancements in food industry and pathogen detection methods. The development of a rapid, highly sensitive and selective biosensor for pathogen detection in foodstuffs is necessary to both protect human health and prevent economic losses. In this project, the specific interaction between an antibody and its corresponding pathogen bacteria was utilized to generate a detection assay using surface enhanced Raman scattering (SERS). In order to obtain a SERS signal, silver nanoparticles and silver coated magnetic nanoparticles were used in combination with antibodies. To develop the biosensor, initially the immobilisation of the antibody onto the surface of the silver nanoparticle was optimised by adopting the PEGylation procedure which is one of the covalent immobilisation methods. In this procedure, the thiol group of the PEG molecule was attached to the nanoparticle surface, while the amine group was linked to the carboxyl group of the antibody. Then the co-functionalisation of nanoparticles with both antibodies and a Raman reporter was developed to determine optimum conditions. In order to determine these conditions, a range of FITC concentrations, PEG solutions and antibody solutions were analysed using UV-Vis and Raman Spectroscopy. In a presence of a target and external magnetic field, an enhanced Raman signal was observed due to controlled aggregation of nanoparticles and presence of a Raman reporter dye.

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