Regulation of the Drosophila initiator caspase dronc through ubiquitylation
Başlık çevirisi mevcut değil.
- Tez No: 602326
- Danışmanlar: DR. ANDREAS BERGMANN
- Tez Türü: Doktora
- Konular: Biyomühendislik, Tıbbi Biyoloji, Bioengineering, Medical Biology
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2017
- Dil: İngilizce
- Üniversite: University of Massachusetts Medical School
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: 212
Özet
Özet yok.
Özet (Çeviri)
Apoptosis is a programmed cell death mechanism that is evolutionary conserved from worms to humans. Apoptosis is mediated by initiator and effector caspases. The initiator caspases carry long pro-domains for their interaction with scaffolding proteins to form a cell-death platform, which is essential for their activation. Activated initiator caspases then cleave effector caspases that execute cell death through cleaving downstream targets. In addition to their apoptotic function, caspases also participate in events where caspase activity is not required for cell killing, but for regulating other functions, so-called non-apoptotic functions of caspases. The Drosophila initiator caspase Dronc, the ortholog of mammalian caspase-2 and caspase-9 has a CARD domain that is essential for its interaction with the scaffolding protein Dark to form the apoptosome. Apoptosome formation is crucial for activation of Dronc. Activity of both initiator and effector caspases are further kept in control by the ubiquitin system to avoid inappropriate caspase activity. However, mechanistic details of how the ubiquitin system regulates activation of Dronc are not clear. Therefore, I investigated the ubiquitylation status of Dronc and its function in Drosophila. I found that Dronc is mono-ubiquitylated at Lys78 (K78) in its CARD domain, which blocks its interaction with Dark and formation of the apoptosome. Furthermore, I demonstrated that K78 mono-ubiquitylation plays an inhibitory role in Dronc's non-apoptotic functions, which may not require its catalytic activity but may be important for the survival of the fly. This thesis study unveils the link between the ubiquitin system and caspases through a regulatory mechanism where a single mono-ubiquitylation event could inhibit both apoptotic and non-apoptotic functions of a caspase.
Benzer Tezler
- Histone mRNA translation in metazoans: SLIP1 as the bridging factor between the 5' and 3' utrs of the histone mRNA
Başlık çevirisi yok
NİHAL GÜLSEREN ÇAKMAKÇI
- Cloning and initial characterization of an estrogen responsive gene: YPEL2
Östrojen yanıt geni YPEL2'nin klonlanması ve proteinin ilkin karakterizasyonu
GİZEM GÜPÜR
Yüksek Lisans
İngilizce
2014
BiyolojiOrta Doğu Teknik ÜniversitesiBiyoloji Ana Bilim Dalı
DOÇ. DR. MESUT MUYAN
- Genomic profiling of microRNAs regulating translation in Drosophila melanogaster embryos
Drosophila melanogaster embriyolarında translasyonu düzenleyen mikroRNAların genomik profillenmesi
ÖZGE TÜNCEL
Yüksek Lisans
İngilizce
2008
Biyolojiİzmir Yüksek Teknoloji EnstitüsüMoleküler Biyoloji ve Genetik Ana Bilim Dalı
YRD. DOÇ. DR. BÜNYAMİN AKGÜL
- Development of a Drosophila CMT4A model using IMAGO approach
İAGSY yaklaşımını kullanarak bir CMT4A Drosophila modelinin geliştirilmesi
KEREM YILDIRIM
Yüksek Lisans
İngilizce
2013
BiyolojiBoğaziçi ÜniversitesiMoleküler Biyoloji ve Genetik Ana Bilim Dalı
PROF. DR. ESRA BATTALOĞLU
DOÇ. DR. ARZU ÇELİK FUSS
- Drosophila melanogaster'de PGRP-LF geninin otoinflamasyon bakımından etkileştiği diğer genlerin genom çaplı ilişkilendirme modeliyle saptanması
Determining the other genes interacting with the autoinflammation related pPGRP-LF gene of Drosophila melanogaster via genome wide association study
HAZAL ÇALIŞKAN
Yüksek Lisans
Türkçe
2019
BiyolojiHacettepe ÜniversitesiBiyoloji Ana Bilim Dalı
PROF. DR. ERGİ DENİZ ÖZSOY