Circadian regulation of pathogenicityin the biotrophic downy milde wHyaloperonospora arabidopsidis
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- Tez No: 716617
- Danışmanlar: Belirtilmemiş.
- Tez Türü: Doktora
- Konular: Biyoloji, Biyomühendislik, Biology, Bioengineering
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2019
- Dil: İngilizce
- Üniversite: Unıversıty Of Worcester
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: Belirtilmemiş.
Özet
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Özet (Çeviri)
Most organisms have an internal biological clock that also called the circadian rhythm. The clock is synchronized by the day–night cycle, allowing the organism to accommodate the daily cycles of light and dark attributable to the Earth's rotation. The circadian clocks have three basic properties; a period length of about 24 hours, can be reset by environmental factors such as light and temperature, and have at least one internal autonomous circadian oscillator. These oscillators contain positive and negative elements that form auto-regulatory feedback loops, and in many cases these loops are used to generate 24-hour timing circuits. The identification of clock-regulated genes (CRGs) has led to the determination of common elements that regulate these genes. If these common elements can also be identified in pathogens, this would give us information on whether virulence factors are also regulated by the circadian rhythm. Recently, a link between the plant immune system and the circadian clock has been identified and, therefore, it is imperative that we identify the link between the pathogenicity factors and the circadian rhythm. For this investigation, the model plant Arabidopsis thaliana (At) and its native pathogen Hyaloperonospora arabidopsidis (HPA), the causative agent of the downy mildew disease, were used. The main aims were to determine whether HPA is affected by the circadian rhythm and, if so, how the pathogen regulates this rhythm; whether the rhythm is important for its pathogenicity, and more importantly whether selected virulence factors show circadian regulated rhythmic expression patterns. At the pathology level, it has been observed that HPA's germination, development and sporulation phases are affected under irregular light regimes, and continuous light and darkness suppress the development phase of HPA. This indicates that the light is vital for HPA development. At the molecular level, it was clear that 2 genes play an effective role in HPA circadian regulation. These genes were designated HPA-TIM and HPA-SRR1, and their expression patterns were totally similar to At-CCA1 and At-LHY. This data was the first to demonstrate that biological clock genes can show synchronization in plant-pathogen relationships in oomycetes. It was also stated for the first time in this thesis that the expression patterns of ATR1 and ATR13, two of the cloned HPA effector genes, are compatible with circadian regulation and peak points were recorded when the plant immunity was at the highest period. In addition, relations between HPA and At CRGs were investigated and it was revealed that mutations in plant CRGs had effects not only on the plant immunity, but also on the HPA CRGs expressions and pathogenicity. In the double mutants of CCA1, LHY and TOC1, which are the three-basic CRGs for Arabidopsis, HPA sporulation was decreased and sporulation was totally suppressed in the triple mutant line. This data also shows us that there is a possible link between Arabidopsis and HPA circadian mechanisms, and the absence of clock genes in the plant affects circadian regulation of HPA, thereby affecting pathogenicity. In the final part, two gene silencing methods HIGS (Host-induced gene silencing) and application of small RNA (sRNA) (which is also named as SIGS; Sprayed induced gene silencing) were used. While HIGS system was unsuccessful, the Hpa-CesA3 gene was silenced successfully using antisense sRNA.
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