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Controlling magnetoelectric nanoparticles for wireless stimulation and recording of neuronswith electromagnetic systems

Nöronların kablosuz stimülasyonu ve kaydı için manyetoelektrik nanoparçacıkların elektromanyetik sistemlerle kumanda edilmesi

  1. Tez No: 770049
  2. Yazar: YAGMUR AKİN YİLDİRİM
  3. Danışmanlar: PROF. DR. SAKHRAT KHİZROEV
  4. Tez Türü: Doktora
  5. Konular: Elektrik ve Elektronik Mühendisliği, Electrical and Electronics Engineering
  6. Anahtar Kelimeler: Belirtilmemiş.
  7. Yıl: 2022
  8. Dil: İngilizce
  9. Üniversite: University of Miami
  10. Enstitü: Yurtdışı Enstitü
  11. Ana Bilim Dalı: Elektrik ve Bilgisayar Mühendisliği Ana Bilim Dalı
  12. Bilim Dalı: Belirtilmemiş.
  13. Sayfa Sayısı: 131

Özet

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Özet (Çeviri)

Recently, Magnetoelectric nanoparticles (MENPs) have been introduced as a potential enabler of a wireless brain-machine interface (BMI). MENPs convert externally applied magnetic fields into electric dipole fields, which in turn can stimulate neurons without the need for invasive electrodes. Both stimulation and recording based on MENPs obey novel underlying physical principles which are quite different from the traditional stimulation approaches, e.g., the popular intracortical microstimulation (ICMS) based on using microelectrodes. In ICMS, electric currents were flown between two microelectrodes to excite neurons; as a result, the stimulation region is defined by the current propagation region and thus is relatively large. In contrast, with MENPs, the stimulation is due to the electric fields (not currents) by individual nanoparticles. These electric fields are localized in the 50-nm proximity range around activated nanoparticles. As for the nanoparticles' activation process, the physical properties of the nanoparticles, such as the magnetic coercivity are used. So far, the main challenges with this approach have been to provide sufficiently high magnetic-to-electric field coupling to locally depolarize the resting membrane potential of neurons, thus evoking action potentials, and to engineer an electromagnetic system capable of testing these novel MENPs-based write/read approaches. In this dissertation, we addressed these challenges and successfully developed several electromagnetic systems designs capable of controlling the properties of MENPs, thus enabling wireless writing and recording processes. Herein, ~30 nm polyethylene glycol (PEG) coated MENPs consisting of a magnetostrictive core (CoFe2O4) and piezoelectric shell (BaTiO3) with a coercivity field of ~300 Oe have been used in E18 rat hippocampal cell culture of ~100,000 neurons. Neuronal cultures were loaded with Cal-520 (fluorescent Ca2+ sensitive indicator) to do an optical read-out of action potentials. When stimulated, MENPs were shown to wirelessly induce calcium spikes which were synchronized with the application of 2-seconds-long trains of ~1200 Oe bipolar magnetic pulses from an electromagnet driven by an audio amplifier at the rate of 20 pulses/sec, with a pulse width of 25 msec. The observed calcium spikes were compared with the traditional electrical stimulation with a 50 μA current applied by two silver electrodes placed in the cell culture and showed similar spikes in terms of shape and magnitude. Also, a common control study was made with sodium channel blocker tetrodotoxin (TTX) and no neural activity was observed after adding TTX. As expected according to the nanoparticles' coercivity driven on/off operation, MENPs operated below their coercivity value of 300 Oe failed to induce synchronized calcium spikes. Furthermore, localization and channelization were demonstrated with a special two-headed electromagnet design in vitro as well as in vivo (rats) with sub-50-ms temporal resolution. Moreover, the physics of recording neural activity with MENPs is explained, and preliminary in vitro experiments successfully proved the theory. Research is ongoing on this topic to collect more statistically significant data. Overall, this dissertation provides evidence for MENPs-induced non-invasive and wireless stimulation and recording of neural activities with high temporal and spatial resolution

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